BioLegend’s Go-ChIP-Grade™ kit offers all the major components required for completing your ChIP assay from cell collection to immunoprecipitation and DNA purification. The kits feature spin columns based on solid-state technology from Chromatrap® and are suitable for ChIP assays from as little as 1 µg to up to 50 µg of protein. Our kit has been QC tested and validated with ChIP-qPCR.

Chromatrap’s Technology: This patented format is unique and has been granted in the UK (Patent No. GB2482209), the US (Patent No. 9523681), China (Patent No. ZL 2011 8 0067254.X) Japan (Patent No. JP 6088434) and Australia (Patent No. AU 2011340263).

Advantages of the kit:

  • The kit is compatible for use with ChIP-qPCR, ChIP-on-chip, and ChIP-seq assays
  • Suitable for ChIP assays using as little as 1 µg and to up to 50 µg of chromatin
  • ChIP assay can be completed in 1-2 days
  • Column contains inert polymer disc, reducing non-specific binding
  • Provides reduced immunoprecipitation (IP) incubation times
  • Elution chemistry is optimized for high quality and quantity of immunoprecipitated (IP'ed) DNA

 

Go-ChIP-Grade™ Protein G Enzymatic Kit

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Go-ChIP-Grade™ Protein G Enzymatic Kit provides a sensitive, reliable, and efficient method for enzymatic Chromatin IP. The kit is complete with all reagents and buffers necessary for ChIP assay and it revolutionizes the use of spin columns for ChIP assay. Each column contains a disc of an inert, porous polymer to which protein G has been covalently attached. During the assay, the target chromatin/antibody complex is retained by the disc. Flushing with three wash buffers and an elution step are all that is required to obtain the DNA fragments of interest. 

Go-ChIP-Grade™ Protein G Enzymatic Kit is preferable to other methods because:

  • Go-ChIP-Grade™ Protein G Enzymatic Kit columns eliminate the needs for beads.
  • Go-ChIP-Grade™ Protein G Enzymatic Kit columns contain a proprietary porous polymer disc to which protein G has been covalently anchored. Its pores have inner surfaces specifically designed to maximize chromatin capture efficiency.
  • The disc base material is chemically inert, reducing incidences of non-specific binding.
  • All chromatin capture takes place inside the disc, therefore there is no dependence on risky pipette separation procedures.
  • The standard protocol does not require pre-blocking and pre-wash steps.
  • Flushing away unbound chromatin and other unwanted material is easily achieved by using washing steps that are fast and less prone to error.
     

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