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DNA Dyes

Using DNA Dyes to Assess the Cell Cycle

 

One of the most common ways to visualize the cell cycle is by using DNA dyes that bind stoichiometrically to dsDNA. Cell permeant dyes like Cytophase™ Violet and DRAQ5™ can measure the cell cycle in live cells while membrane impermeant dyes like Propidium Iodide, DRAQ7™, and Helix NP™ can only assess cell cycle status in cells that have been fixed or permeabilized.

 

Learn more about DNA dyes

 

To more accurately measure the number of cells in S phase, BrdU can be used in addition to a DNA dye. BrdU is incorporated into DNA during S-phase of the cell cycle as a thymidine substitute. Cells can be pulse-labeled with BrdU and any cells in S-phase during that time interval will be positive when stained with an anti-BrdU antibody.

CytoPhase™ Violet

 

CytoPhase™ Violet is a cell-permeant DNA-binding dye that can be excited by either the UV (355 nm or 375 nm) or violet laser (405 nm). It can be used for flow cytometric analysis of the cell cycle in live or fixed cells, and can be used concurrently with antibody staining procedures.

 

Live Ramos cells stained with 5 µM CytoPhase™ Violet. A typical cell cycle profile will contain two distinct peaks representing cells in G0/G1 and G2/M. Cells that fall between these peaks are in S-phase.

Phase-Flow™ BrdU Kits

 

Phase-Flow™ kits are designed for streamlined analysis of BrdU incorporation by flow cytometry. The kits provide BrdU pulsing solution, anti-BrdU antibody, all necessary buffers, and DNA dyes DAPI and 7-AAD.

 

 

Ramos cells loaded with BrdU for 1.5 hours (left) or as no load controls (right) stained using the Phase-Flow™ FITC BrdU Kit and DAPI.​

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