Focus on a single target or measure up to 13 at once with BioLegend immunoassays. Find all of the reagents for your ELISA workflow with our economical kits. Measure multiple soluble proteins simultaneously with pre-defined or customized LEGENDplex™ multiplex assays. Whether your goals are specific or broad, our solutions streamline your path to discovery.

BioLegend's LEGENDplex™ bead-based immunoassays quantify multiple soluble analytes simultaneously in biological samples using a flow cytometer. The assay utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies.

 

 

Each kit provides sufficient reagents for at least 100 tests. The assay can be performed in a 96-well filter plate or v-bottom plate, allowing you to run 40 samples plus 8 standard curve titrations in duplicate.

 

Our LEGENDplex™ products include:

 

Pre-defined Panels: Choose from dozens of existing panels, ranging from 3 to 13 targets chosen to study specific pathways like inflammation, anti-viral response, or immune checkpoint molecules.

 

Mix and Match Panels: Use the mix and match system if you are interested in ordering a subset of specificities from one of our pre-defined panels.

 

Custom Panels: Design custom panels to combine analytes from multiple panels or request analytes that are not currently in our catalog. Each request will be evaluated for feasibility on a case-by-case basis.

 

Software: Data analysis software is free. Choose from a cloud-based program or downloadable program.

 

For increased flexibility and convenience, we offer four types of ELISA Kits for both experts and beginners. Each of our ELISA Kits are designed for the quantifications of proteins in biological samples including cell culture supernatant, serum, and plasma. They offer high accuracy, sample linearity, and testing on biologically relevant samples.

 

RAPID MAX™ ELISA Kits cut assay time down to less than 90 minutes. Each kit is fully validated and contains a 96-well strip plate that has been pre-coated to immobilize the capture antibody.

 

LEGEND MAX™ ELISA Kits are fully validated and ready-to-use, containing all required reagents including a 96-well strip plate pre-coated with capture antibody.

 

ELISA MAX™ Deluxe Sets are a more cost effective solution and contain pre-optimized reagents with some additional buffers/solutions.

 

ELISA MAX™ Standard Sets provide only the basic components to complete an ELISA, including a recombinant protein standard, and capture and detection antibodies.  

 

 

Components

RAPID MAX™ Kit

LEGEND MAX™ Kit

ELISA MAX™ Deluxe Set

ELISA MAX™ Standard Set

Assay Time

90 mins

4 hours

Overnight + 6 hours

Overnight + 6 hours

Experience Level

Beginner-Advanced

Beginner-Advanced

Intermediate - Advanced

Intermediate - Advanced

Strip-Well Plate

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Capture Antibody

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Pre-coated on plate

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Detection Antibody

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Recombinant Protein Standard

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Avidin-HRP

Not required

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Coating Buffer

Not required 

Not required

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Wash Buffer

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Assay Buffer/Assay Diluent/Matrix

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Substrate/TMB Solution

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Stop Solution

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Plate Sealers

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Antibodies

 

BioLegend provides an extensive selection of purified and conjugated antibodies against human, mouse, and rat soluble proteins. We offer antibodies for use in a variety of applications including ELISA, flow cytometry, western blotting, and functional assays.

 

Browse by species reactivity:

 

Human Mouse Rat
Antibodies to Cytokines and Chemokines Antibodies to Cytokines and Chemokines Antibodies to Cytokines and Chemokines
Antibodies to Receptors Antibodies to Receptors ELISA Antibody Pairs
ELISA Antibody Pairs ELISA Antibody Pairs  

 

Browse biomarker antibodies by application:

Recombinant Protein Standards

 

Having the correct standards is critical for the accuracy and dependability of an ELISA assay. BioLegend provides lyophilized recombinant protein standards, optimized for use with our ELISA pairs.

 

Browse products by species below:

 

We also provide recombinant cytokines, chemokines, and growth factors for bioassays or cell/tissue culture.

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