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Workflow and Troubleshooting

Ce3D™ Tissue Clearing Kit Staining Protocol for Microscopy

 

View our step-by-step protocol video
for Ce3D™ reagents.

Our protocol and troubleshooting guide provides an in-depth optimized protocol, complete with notes and commentary for each step. The guide also outlines the entire procedure, leading you through perfusion, fixation, sectioning, staining, tissue clearing, and mounting steps. The process itself can typically be completed within 7 days.

 

 

In addition, the guide contains suggestions for resolving potential issues you may encounter as you optimize your experiment. Read through it for tips on fixation, uneven staining, and high background. For additional information or help, contact our Technical Services team.

Recommended Clearing Time for Different Tissues

 

Time Tissue
2 hours Intestine, lymph node
4 hours Spleen, thymus, lung
> 8 hours Kidney, liver, testis, brain

Ce3D™ Tissue Clearing Workflow

 

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