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Ce3D Tissue Clearing

What is Ce3D™?

 

Ce3D™ (clearing-enhanced 3D) Tissue Clearing Reagent is a hydrophilic and RI-matching solution, making it easy-to-use for the rapid and robust clearing of diverse tissues. Ce3D™ has an RI of ~1.5 and is compatible with multicolor fluorescence microscopy. The Ce3D™ tissue clearing technique works by simple immersion of tissue into the clearing reagent, and does not require special equipment or containers. Ce3D™ offers many advantages:

 

Key Features

 

  • Simple, one-step passive clearing process
  • Preservation of fluorescent signal, tissue architecture, and cell morphology
  • Reversible to allow 2D IHC and other downstream applications
  • Available as pre-assembled kits or as individual reagents, saving time on buffer preparation
  • Validated with over 60 BioLegend antibody clones and conjugated formats

Ce3D™ Tissue Clearing Kits

 

Test Size Cat. No. Ce3D™ Tissue Clearing Solution Ce3D™ Permeabilization/Blocking Buffer Ce3D™ Antibody Diluent Buffer Ce3D™ Wash Buffer
10 Tests 427701 5 mL 15 mL 15 mL 50 mL
50 Tests 427702 25 mL 50 mL 50 mL 250 mL

 

 

Ce3D™ Tissue Clearing Individual Reagents

 

Reagent Size (µL) Cat. No.
Ce3D™ Tissue Clearing Solution 5 mL | 25 mL 427703 | 427704
Ce3D™ Permeabilization/Blocking Buffer 25 mL | 50 mL 427705 | 427706
Ce3D™ Antibody Diluent Buffer 15 mL | 50 mL 427707 | 427708
Ce3D™ Wash Buffer 50 mL | 250 mL 427709 | 427710
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