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The reconstituted Veri-Cells™ Heavy Metal Ta PBMC are intended to offer mass cytometry laboratories stabilized human blood cells which have been labeled with the heavy metal tantalum. When mixed with sample cells suspensions, the cells of interest can be differentiated from the labeled control PBMCs using the tantalum signal with minimal variability across experiments. These cells have been verified to work with commonly tested cell surface markers such as CD3, CD4, CD8, CD14, CD16, CD19, CD38, CD56, CD123 and HLA-DR.
This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative for antibody and nucleic acid testing against human immunodeficiency virus (HIV), Hepatitis B and C virus, HTLV (Human T-lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus, syphilis and West Nile Virus using FDA approved methods. Biological tests are not 100% accurate. Use standard precautions when handling, and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.
- Kit Contents
Cat. No. 427201
- 2 vials of Veri-Cell™ Heavy Metal (Ta) PBMC (approximately 1 X 106 cells per vial)
- 1 vial of 0.7 ml Veri-Cells™ Buffer A
Cat. No. 427203
- 10 vials of Veri-Cell™ Heavy Metal (Ta) PBMC (approximately 1 X 106 cells per vial)
- 5 vials of 0.7 ml Veri-Cells™ Buffer A
Cat. No. 427201: 2 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Cat. No. 427203: 10 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
- The Veri-Cells™ Heavy Metal Ta PBMC kit is prepared from 2 vials of lyophilized human PBMCs labeled with tantalum and a vial of Veri-Cells™ Buffer A.
- Storage & Handling
- Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
FC, CyTOF® - Quality tested
- Recommended Usage
50µL per test (after reconstitution with 325 µL of Veri-Cells™ Buffer A)
- Application Notes
The Veri-Cells™ Heavy Metal Ta PBMC kit contains stabilized human blood cells which have been pre-labeled with the metal tantalum (Ta). The reconstituted human PBMCs can be added to test samples as a control for CyTOF applications. The cells have been verified to work with commonly tested cell markers such as CD3, CD4, CD8, CD14, CD16, CD19, CD38, CD56, CD123 and HLA-DR. Assessing the suitability of this product as a control for the end user’s molecule of interest is recommended. The tantalum signal is confirmed by a CyTOF Helios or equivalent mass cytometry instrument.
Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ Heavy Metal (Ta) PBMC.
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