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Ultra-LEAF™ Purified anti-human CCL19 (MIP-3β) Antibody

Pricing & Availability
Clone
A15093C (See other available formats)
Regulatory Status
RUO
Other Names
MIP-3β, Exodus-3, ELC, CK Beta-11, CKb11, EBI1 Ligand Chemokine, Macrophage Inflammatory Protein 3 Beta
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15093C_ULEAF_CCL19_Antibody_100419
Recombinant Human CCL19 (Cat. No. 582106, black circles) induces the chemotaxis of mouse Baf3-hCCR7 transfectant chemoattractant cells. Anti-human CCL19 antibody (clone A15093C, purple squares) neutralizes the chemotactic activity of recombinant human CCL19 (0.1 µg/mL) on mouse Baf3-hCCR7 cells in a dose-dependent manner while the GoInVivo™ Purified Mouse IgG2b, κ Isotype Ctrl Antibody (Cat. No. 400366, grey triangles) does not have the effect. ND50 range: 0.1 - 1.0 µg/mL.
  • A15093C_ULEAF_CCL19_Antibody_100419
    Recombinant Human CCL19 (Cat. No. 582106, black circles) induces the chemotaxis of mouse Baf3-hCCR7 transfectant chemoattractant cells. Anti-human CCL19 antibody (clone A15093C, purple squares) neutralizes the chemotactic activity of recombinant human CCL19 (0.1 µg/mL) on mouse Baf3-hCCR7 cells in a dose-dependent manner while the GoInVivo™ Purified Mouse IgG2b, κ Isotype Ctrl Antibody (Cat. No. 400366, grey triangles) does not have the effect. ND50 range: 0.1 - 1.0 µg/mL.
  • A15093C_ULEAF_CCL19_Antibody_2_122123
    Direct ELISA showing purified anti-human CCL19 antibody (clone A15093C) bound to plate-immobilized recombinant human CCL19 (MIP-3β) protein (Cat. No. 582102, 582104, or 582106). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
Cat # Size Price Quantity Check Availability Save
612803 100 µg 216€
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612804 1 mg 572€
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Description

CCL19 is a CC chemokine expressed in the lymph nodes. CCL19 is only 32% identical to CCL21 at the amino acid level; nevertheless, these chemokines bind to the same receptor, CCR7.  CCL19 is not expressed in high endothelial venules (HEV) as is CCL21, but CCL19 is transcytosed in HEV. It has been described that CCL19 binds to CCX-CKR and CRAM-B receptors. These two receptors are members of the non-classical chemokine receptor family, which also includes D6, DARC, and CXCR7. It has been suggested that CCX-CKR is a scavenging receptor for CCL19, and the CRAM-B receptor might be responsible for the transcytosis in HEV for CCL19. In addition, CRAM recycles constitutively and can internalize CCL19 within a few minutes, suggesting it may play a role in the regulation of immune responses as well as homing processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CCL19
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CCL19 (0.1 µg/mL) on mouse Baf3-hCCR7 transfectant chemoattractant cells. ND50 range: 0.1 - 1.0 μg/mL. For Direct ELISA, the optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (A15093C) could function as capture antibody when paired with Biotin anti-human CCL19 antibody (Poly5384) as detection antibody using Recombinant Human CCL19 (MIP-3β) protein (Cat. No. 582102, 582104, or 582106) as assay standard.

RRID
AB_2820099 (BioLegend Cat. No. 612803)
AB_2820100 (BioLegend Cat. No. 612804)

Antigen Details

Structure
Chemokine
Distribution

CCL19 is expressed in the T cell zone of lymph nodes, in Peyer's patches by reticular cells, and in the spleen by stromal cells. CCL19 is expressed by endothelial cells in lymphatic venules.

Function
CCL19 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL19 and CCL21 inhibit apoptosis in DCs.
Interaction
Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, antigen-engaged B cells, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA).
Ligand/Receptor
CCR7 (CD197)
Cell Type
B cells, Dendritic cells, Macrophages, T cells
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Rossi DL, et al. 1997. J. Immunol. 158:1033.
  2. Sánchez-Sánchez N, et al. 2004. Blood 104:619.
  3. Comerford I, et al. 2006. Eur. J. Immunol. 36:1904.
  4. Leick M, et al. 2009. Immunology 129:536.
  5. Le Nouën C, et al. 2011. PLoS Pathog. 7:e1002105.
Gene ID
6363 View all products for this Gene ID
UniProt
View information about CCL19 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 12/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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