- Regulatory Status
- Other Names
- Transcription Factor Fixation/Permeabilization Buffer Set
- Ave. Rating
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- Product Citations
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BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.
See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.
Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact.Product Details
- Storage & Handling
- Store between 2°C and 8°C. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.
Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.
NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.
- Application Notes
Buffer Set Contents:
- True-Nuclear™ 4X Fix Concentrate (30 mL)
- True-Nuclear™ Fix Diluent (100 mL)
- True-Nuclear™ 10X Perm Buffer (100 mL)
- Additional Product Notes
View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.
- Product Citations
- Cell Type
- Biology Area
- Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID