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- Regulatory Status
- RUO
- Other Names
- C-type Lectin-like receptor 2 (CLEC2), C-type lectin domain family 1, member B (CLEC1B), CLEC-1b1
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- Product Citations
- publications
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Recombinant mouse CLEC2-Fc chimera, when coated on a plate, is able to bind biotinylated recombinant mouse GP38-Fc protein in a dose dependent manner. The apparent Kd value of this binding is <5.0 nM.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 551804 | 25 µg | 175€ | ||||
| 551806 | 100 µg | 507€ | ||||
CLEC2, also termed CLEC-1B, is a C-type lectin-like receptor. CLEC2 is highly expressed on resting platelets and megakaryocytes, and expressed at lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. The first ligand identified for CLEC2 was rhodocytin, snake venom toxin. Rhodocytin is a strong platelet activator, and this effect is mediated by CLEC2, which has a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (ITAM). Exposure of platelets to rhodocytin leads to tyrosine phosphorylation of the ITAM-like motif and Syk-dependent platelet activation. Podoplanin, also termed GP38 is an endogenous ligand of CLEC2. This molecule was found to be expressed on the surface of tumor cells and facilitates tumor metastasis by inducing platelet aggregation. Podoplanin is also expressed on endothelial cells, and mice deficient in this protein show abnormal patterns of lymphatic vessel formation. In addition, CLEC2 functions as an activating receptor on monoyctes and neutrophils to induce phagocytosis and proinflammatory cytokine production. CLEC2 enhances the expansion of TCR-stimulated T cells by increasing their survival through enhanced expression of anti-apoptotic proteins. Also, CLEC2 modulates the capacity of T cells to home to lymph nodes and stimulates natural killer cell mediated cytotoxicity.
Product DetailsProduct Details
- Source
- Mouse CLEC2, amino acids (Gln57-Leu229) (Accession# NP_064369.1), was expressed in CHO cells with a C-terminal human IgG1 Fc tag.
- Molecular Mass
- The 409 amino acid recombinant protein has a predicted molecular mass of approximately 46.7 kD. The protein migrates at about 60 kD by SDS-PAGE in DTT-reducing conditions and about 120 kD in non-reducing conditions. The predicted N-terminal amino acid is Gln.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 1.0 EU per µg of protein as determine by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant mouse CLEC2, when coated on a plate (2 µg/ml, 100 µl/well) is able to bind biotinylated mouse GP38 in a dose dependent manner with an apparent Kd <5.0 nM.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
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CLEC2 is expressed on platelets, monocytes, macrophages, dendritic cells, and granulocytes.
- Function
- Plays a role in monocyte and neutrophil phagocytosis, modulation of T cell expansion, NK cell cytotoxicity, thrombosis/hemostasis, tumor metastasis, and lymphangiogenesis.
- Interaction
- Tumor cells, fibroblastic reticular cells, lymphatic endothelial cells.
- Ligand/Receptor
- Mouse GP38 (podoplanin), rhodocytin.
- Bioactivity
- Mouse CLEC2 is able to bind mouse GP38.
- Biology Area
- Immunology
- Antigen References
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1. Colonna M, et al. 2000. Eur. J. Immunol. 30:697.
2. Suzuki-Inoue K, et al. 2010. J. Biol. Chem. 285:24494.
3. Herzog BH, et al. 2013. Nature. 502:105.
4. Boulaftali Y, et al. 2013. J. Clin. Invest. 123:908.
5. Manne BK, et al. 2013. J. Biol. Chem. 288:7717.
6. Bénézech C, et al. 2014. Blood. 123:3200. - Gene ID
- 56760 View all products for this Gene ID
- UniProt
- View information about CLEC2 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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