Recombinant Human Endostatin (carrier-free)

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Endostatin_Human_RECOM_BA_092914
Human endostatin inhibits the HUVEC cell proliferation induced by human FGF basic.
  • Endostatin_Human_RECOM_BA_092914
    Human endostatin inhibits the HUVEC cell proliferation induced by human FGF basic.
Cat # Size Price Quantity Avail. Save
556504 25 µg 76€
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556506 100 µg 152€
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Description

Endostatin is a C-terminal proteolytic fragment of collagen XVIII α1 (Col 18) that is localized in the vascular basement membrane of almost all epithelia and endothelia. Endostatin is produced from catalytic cleavage from MMPs, cathepsin L, and S. It is detected in circulation at a physiologic level of 20 to 50 ng/ml in serum. Crystal X-ray analysis of endostatin has shown a compact globular structure, with one face rich in arginine residues that acts as a heparin-binding motif. Endostatin interacts with multiple molecules including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, thrombospondin-1, SPARC, collagens (I, IV, and VI), the amyloid peptide Aβ (1-42), transglutaminase-2, and among others. Endostatins derived from collagens XV and XVIII differ in structure and binding properties, tissue distribution, and anti-angiogenic activity. Endostatin is an endogenous angiostatic factor that induces apoptosis in endothelial cells and inhibits endothelial cell migration in vitro. Col 18-null mice showed faster tumor growth when implanted with tumor cells that do not produce Col 18. Overexpression of circulating endostatin in transgenic mice resulted in reduced tumor growth. Experimental model of glomerulonephritis in Col 18-null mice showed that the absence of this molecule exacerbates immune-mediated glomerulonephritis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human Endostatin, amino acids (His1154-Ser1335) (Accession# NM_130445), was expressed in E. coli. The amino terminal contains a Met-8 His tag.
Molecular Mass
The 192 amino acid recombinant protein has a predicted molecular mass of approximately 21.3 kD. The DTT-reduced and non-reduced protein migrate at approximately 23 and 20 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.5 - 3 µg/ml, as determined by inhibition of HUVEC cell proliferation induced by human FGF basic.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution
Basement membrane of almost all epithelia and endothelia and is present in serum.
Function
Inhibits proliferation, migration, tubule formation of endothelial cells, and angiogenesis and tumor growth. Cathepsins L and S releases two peptides from endostatin with enhanced angiostatic properties.
Interaction
Endothelial cells.
Ligand/Receptor
Integrins (α5β1 and αvβ3), heparan sulfate proteoglycans (glypican-1 and -4), and VEGFR2.
Bioactivity
Inhibition of HUVEC cell proliferation induced by human FGF basic.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Angiogenesis
Antigen References

1. O'Reilly M, et al. 1997. Cell 88:277.
2. Zatterstrom UK, et. al. 2000. Cell Struct. Funct. 25:97.
3. Sund M, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:2934.
4. Faye C, et al. 2009. J. Biol. Chem. 284:22041.
5. Hamano Y, et al. 2010. J. Am. Soc. Nephrol. 21:1445.
6. Veillard F, et al. 2011. J. Biol. Chem. 286:37158.

Gene ID
80781 View all products for this Gene ID
UniProt
View information about Endostatin on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 3    Revision Date: 08/26/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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