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Purified anti-TTF-1 Antibody (Previously Covance catalog# SIG-3830)

Pricing & Availability
Clone
8G7G3/1 (See other available formats)
Regulatory Status
RUO
Other Names
Thyroid Transcription Factor, Homeobox protein Nkx-2.1, thyroid nuclear factor 1, thyroid transcription factor 1 TTF-1 NK-2
Previously
Signet Catalog# 830-26 L2 Predilute
Covance Catalog# SIG-3830
Isotype
Mouse IgG1
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Product Citations
publications
8G7G3-1_PURE_TTF-1_Antibody_1_120619
IHC staining of purified anti-TTF-1 antibody (clone 8G7G3/1) on formalin-fixed paraffin-embedded human lung tumor tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8G7G3-1_PURE_TTF-1_Antibody_1_120619
    IHC staining of purified anti-TTF-1 antibody (clone 8G7G3/1) on formalin-fixed paraffin-embedded human lung tumor tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8G7G3-1_PURE_TTF-1_Antibody_2_120619
    IHC staining of purified anti-TTF-1 antibody (clone 8G7G3/1) on formalin-fixed paraffin-embedded rat lung tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
918101 6 mL 246€
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Description

Transcription termination factor-1 (TTF-1) plays a critical role in ribosomal gene transcription. This transcription factor mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. It binds to and activates the promoter of thyroid specific genes such as thyroglobulin, thyrotropin receptor, and thyroperoxidase. TTF-1 is important in the maintenance of the thyroid differentiation phenotype.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using a rat TTF-1 recombinant protein.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, the antibody is ready-to-use.

Application Notes

The BioLegend TTF-1 (8G7G3/1) antibody recognizes the 40kD thyroid transcription factor, which is a member of the NKx2 family of transcription factors. TTF-1 antigen is expressed in epithelial cells of the thyroid gland and the lung.

RRID
AB_2565205 (BioLegend Cat. No. 918101)

Antigen Details

Biology Area
Cell Biology, Signal Transduction
Gene ID
25628 View all products for this Gene ID
UniProt
View information about TTF-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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