- BL26881 (See other available formats)
- Regulatory Status
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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S-tag is the name of an oligopeptide derived from pancreatic RNase A. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. The S-Tag sequence is a fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using affinity separation method.Product Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Host Species
- Synthetic Peptide
- Liquid in phosphate-buffered solution containing 50% glycerol, 0.25% BSA and 0.02% sodium azide.
- The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2629593 (BioLegend Cat. No. 688102)
- The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser.
Derived from pancreatic ribonuclease A (RNase A).
- S-tag can be attached to the N- or C-terminus of protein to become S-tag fused protein.
- Biology Area
- Cell Biology
- Antigen References
1. Raines RT, et al. 2000. Methods Enzymol. 326:362.
- Gene ID
- View information about S-Tag on UniProt.org
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