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Purified anti-PDHA1 Antibody

Pricing & Availability
Clone
3H2-F8-B5 (See other available formats)
Regulatory Status
RUO
Other Names
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, Pyruvate Dehydrogenase E1 Subunit Alpha 1, Pyruvate Dehydrogenase (Lipoamide) Alpha 1, E1alpha
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a_3H2_F8_B5_PURE_PDHA1_Antibody_WB_020723
Whole cell extracts (15 µg total protein) from HeLa, HepG2, SH-SY5Y, and NIH/3T3 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL purified anti-PDHA1 (clone 3H2-F8-B5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • a_3H2_F8_B5_PURE_PDHA1_Antibody_WB_020723
    Whole cell extracts (15 µg total protein) from HeLa, HepG2, SH-SY5Y, and NIH/3T3 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL purified anti-PDHA1 (clone 3H2-F8-B5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • b_3H2_F8_B5_PURE_PDHA1_Antibody_ICC_020723
    ICC staining of purified anti-PDHA1 (clone 3H2-F8-B5) on HeLa cells. Cells were co-stained with 100 nM of MitoSpy™ Red CMXRos (Cat. No. 424801) (panel A) for 20 minutes at 37°C, fixed with ice-cold methanol, then blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with purified mouse IgG1, κ isotype control (Cat. No. 400102) (panel D) or with 1.25 µg/mL anti-PDHA1 (clone 3H2-F8-B5) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) (panel B) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Panel C is an overlay image of Panel A and B and DAPI counterstain. Images were captured with a 60X objective. Scale bar: 20 µm
  • c_3H2_F8_B5_PURE_PDHA1_Antibody_IHC_020723
    IHC staining of purified anti-PDHA1 (clone 3H2-F8-B5) on formalin-fixed paraffin-embedded human cortex tissue. Following antigen retrieval using Tris-EDTA buffer (10 mM Tris, 1mM EDTA, pH 9.0), the tissue was incubated with 1 μg/mL of the primary antibody (panel B) or secondary only control (panel A) overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801) and images were captured with a 40X objective. Scale bar: 50 μm.
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610951 25 µg 96€
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610952 100 µg 296€
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Description

PDHA1, pyruvate dehydrogenase E1 subunit alpha 1, is a major mitochondrial component of pyruvate dehydrogenase complexes (PDCs) which catalyzes oxidative decarboxylation of pyruvate in glycolysis and produces acetyl-CoA for the tricarboxylic acid (TCA) cycle. PDHA1 and PDHB form the heteroterameric pyruvate dehydrogenase E1 (E1) subunit of the PDC. The enzymatic activity of the PDC is regulated by phosphorylation of PDHA1 through pyruvate dehydrogenase kinase (PDK) and pyruvate dehydrogenase phosphatases (PDP). PDCs play crucial physiological functions between fed and fasting states which regulate pyruvate metabolism to maintain glucose homeostasis. PDHA1 enzymatic dysfunction causes fatal lactic acidosis and chronic neurologic dysfunction in the central nervous system. The decreased expression of PDHA1 in Parkinson’s disease and dementia with Lewy bodies patients suggests a dysfunction of mitochondrial energy production. Additionally, dysregulation of PDHA1 is found in multiple cancers, including lung, ovarian, stomach, and kidney, suggesting a role for PDHA1 in tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human PDHA1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The suggested ICC fixation/permeabilization method is 4% paraformaldehyde plus methanol or ice-cold methanol only. Permeabilization with Triton X-100 is not recommended.

For immunohistochemistry, the suggested antigen retrieval buffer is Tris-EDTA pH 9.0.

RRID
AB_2936739 (BioLegend Cat. No. 610951)
AB_2936739 (BioLegend Cat. No. 610952)

Antigen Details

Structure
PDHA1 is a 390 amino-acid protein with a predicted molecular weight of 43.3 kD that forms a heterotetramer with PDHB.
Distribution

Ubiquitous/mitochondria

Function
A subunit of pyruvate dehydrogenase E1 catalyzing the oxidative decarboxylation of pyruvate with the formation of acetyl-CoA and CO2, as part of pyruvate dehydrogenase complex for TCA cycle
Interaction
Forms a heteroteramer with PDHB; interacts with other pyruvate dehydrogenase complex components, E2 and E3.
Biology Area
Mitochondrial Function, Neurodegeneration
Molecular Family
Enzymes and Regulators, Mitochondrial Markers, Organelle Markers
Antigen References
  1. Zhang C, et al. 2011. Proc Natl Acad Sci U S A. 108:16259-64.
  2. Patel MS, et al. 2014. J Biol Chem. 289:16615-23.
  3. Sperl W, et al. 2014. J Inherit Metab Dis. 38:391-403.
  4. Miki Y, et al. 2017. Biochem Biophys Res Commun. 489:439-444.
  5. Deng L, et al. 2022. Front Pharmacol. 13:947372.
Gene ID
5160 View all products for this Gene ID
UniProt
View information about PDHA1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/07/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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