Purified anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119
C57BL/6 bone marrow cells stained with TER-119 FITC and CD45 PE
  • TER-119
    C57BL/6 bone marrow cells stained with TER-119 FITC and CD45 PE
  • Ter-119_pure_Ter119Erythroid_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified TER-119/Erythroid Cells clone TER-119 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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116201 50 µg 34€
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116202 500 µg 111€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IHC-P, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  2. Baganha F, et al. 2022. Int J Mol Sci. 23:. PubMed
  3. Chamberlin T, et al. 2020. Cancer Research. 80(20):4465-4475. PubMed
  4. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
  5. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
  6. Yanagida K, et al. 2020. Dev Cell. 779:52. PubMed
  7. Chen L, et al. 2019. Nat Commun. 3.553472222. PubMed
  8. Motoyama S, et al. 2020. Cells. :9. PubMed
  9. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  10. Peng V, et al. 2020. J Biol Chem. 295:14866. PubMed
  11. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  12. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  13. Blume J, et al. 2019. Eur J Immunol. 49:121. PubMed
  14. Heuser M, et al. 2007. Blood. 110:1639. PubMed
  15. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  16. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  17. Brown IK, et al. 2021. PLoS Pathog. 17:e1009602. PubMed
  18. Chappaz S, et al. 2007. Blood. 110:3862. PubMed
  19. Niaudet C, et al. 2019. Angiogenesis. 1.257638889. PubMed
  20. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  21. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  22. You X, et al. 2022. Front Genet. 12:790990. PubMed
  23. Tahara N, et al. 2018. Dev Biol. 434:74. PubMed
  24. Duong CN, et al. 2020. Arterioscler Thromb Vasc Biol. 40:378. PubMed
  25. Malik N, et al. 2019. Sci Rep. 9:16917. PubMed
  26. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  27. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  28. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  29. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  30. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  31. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  32. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  33. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  34. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  35. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
  36. Cheng P, et al. 2008. J Exp Med. 205:2235. PubMed
  37. Gui J, et al. 2007. Int Immunol. 1.625694444. PubMed
  38. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  39. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
  40. Gough S, et al. 2014. Cancer Discovery. 4:564. PubMed
RRID
AB_313702 (BioLegend Cat. No. 116201)
AB_313703 (BioLegend Cat. No. 116202)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All TER-119 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TER-119/Erythroid Cells TER-119 FC
Biotin anti-mouse TER-119/Erythroid Cells TER-119 FC
FITC anti-mouse TER-119/Erythroid Cells TER-119 FC
PE anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready) TER-119 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody TER-119 FC
Go To Top Version: 4    Revision Date: 04/23/2020

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse TER-119/Erythroid Cells

  • Biotin anti-mouse TER-119/Erythroid Cells

  • FITC anti-mouse TER-119/Erythroid Cells

  • PE anti-mouse TER-119/Erythroid Cells

  • PE/Cyanine5 anti-mouse TER-119/Erythroid Cells

  • Purified anti-mouse TER-119/Erythroid Cells

  • Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells

  • Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells

  • Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells

  • PE/Cyanine7 anti-mouse TER-119/Erythroid Cells

  • APC/Cyanine7 anti-mouse TER-119/Erythroid Cells

  • PerCP anti-mouse TER-119/Erythroid Cells

  • PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells

  • Pacific Blue™ anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells

  • Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells

  • TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells

  • APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells

  • TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells

  • TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells

  • Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells

  • Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells

  • APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells

  • Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody

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