Purified anti-LEF1 Antibody

Pricing & Availability
Clone
15H5A18 (See other available formats)
Other Names
Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha (TCF1-alpha)
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_15H5A18_PURE_LEF1_Antibody_WB_121217
Total lysates (15 µg protein) from U87-MG (Low expression negative control) and Jurkat (Positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-LEF-1 antibody, clone 15H5A18. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (# 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (# 643807, 1:8000 dilution).
  • 1_15H5A18_PURE_LEF1_Antibody_WB_121217
    Total lysates (15 µg protein) from U87-MG (Low expression negative control) and Jurkat (Positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-LEF-1 antibody, clone 15H5A18. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (# 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (# 643807, 1:8000 dilution).
  • 2_15H5A18_PURE_LEF1_Antibody_2_IP_060716
    Immunoprecipitation of LEF1 from Jurkat cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-LEF1 antibody (clone 15H5A18, lane 4). Western blot was performed using anti-LEF1 antibody (clone 15H5A18).
Cat # Size Price Quantity Avail. Save
653101 25 µg 68€
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653102 100 µg 172€
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Description

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human LEF1 recombinant protein (1-298 aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-1.0 µg per ml (1:5000 - 1:500). For immunoprecipitation, the suggested use of this reagent is 5-20 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

LEF-1 is known to have 7 isoforms.

RRID
AB_2561614 (BioLegend Cat. No. 653101)
AB_2561615 (BioLegend Cat. No. 653102)

Antigen Details

Structure
44 kD protein containing a DNA-binding domain (HMG box), an CTNNB1-binding region, and a Prolin-rich domain
Distribution

Nucleus

Function
LEF1 is a transcription factor mediating canonical Wnt signaling pathway. It has been shown to function in hair bud formation, cancer development, tumorigenesis, angiogenesis, and T-cell maturation.
Interaction
Interacts with ALX4, AML-1, beta-catenin (CTNNB1), EP300, MITF, PIAS4, SMAD2, and SMAD3
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
TCRs, Nuclear Markers
Antigen References

1. Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184.
2. Waterman ML, et al. 1991. Genes Dev. 5:656.
3. Hovanes K, et al. 2001. Nat Genet. 28:53.
4. Bruhn L, et al. 1997. Genes Dev. 11:640.
5. Nguyen A, et al. 2005. Int. J. Oncol. 27:949.
6. Petropoulos K, et al. 2008. J. Exp. Med. 205:515.

Gene ID
51176 View all products for this Gene ID
UniProt
View information about LEF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 02/13/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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