Purified anti-human Podocalyxin Antibody

Pricing & Availability
Clone
mAb 84 (See other available formats)
Other Names
GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1, GCTM2
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
mAb_84_PURE_Podocalyxin_Antibody_090117
iPS cells (NC6) were stained with purified PODXL (clone mAb 84, filled histogram) or mouse IgM, κ isotype control (open hisotgram), followed by anti-mouse IgM PE.
  • mAb_84_PURE_Podocalyxin_Antibody_090117
    iPS cells (NC6) were stained with purified PODXL (clone mAb 84, filled histogram) or mouse IgM, κ isotype control (open hisotgram), followed by anti-mouse IgM PE.
  • mAb_84_PURE_Podocalyxin_Antibody_Dot_1_081817
    Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 APC and purified PODXL (clone mAb 84, left) or mouse IgM, κ isotype control (right), followed by anti-mouse IgM PE.
  • mAb_84_PURE_Podocalyxin_Antibody_Dot_2_081817
Cat # Size Price Quantity Avail. Save
374302 100 µg 184€
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Description

Podocalyxin-like protein-1 (also known as PODXL, GP200, PCLP1 and GCTM2) is expressed on the surface of undifferentiated human ES cells and glomerular podocytes. PODXL was originally described as the main sialoprotein expressed by glomerular epithelial cells. Recently PODXL been associated with several forms of cancer and is being targeted as a possible therapeutic. Clone mAb 84 is reported to recognize and have cytotoxic affects against undifferentiated human ES cells. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Cyt, IF, IP, WB - Reported in the Literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: WB, IP, cytotoxicity. The LEAF purified format is recommended for cytotoxicity assays. 

RRID
AB_2715813 (BioLegend Cat. No. 374302)

Antigen Details

Structure
Sialomucin family
Distribution

Stem cells, Glomerular podocytes

Function
Stem cell differentiation, regulatory factor
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Antigen References

1. Kerjaschki D, et al. 1984. J Cell Bio. 98:1591.
2. Horvat R, et al. 1986. J Cell Bio. 102:484.
3. Choo AB, et al. 2008. Stem Cells. 26:1454.
4. Tan HL, et al. 2009. Stem Cells. 27:1792.
5. Nielsen JS, et al. 2009. J Am Soc Nephrol. 20:1669.

Gene ID
5420 View all products for this Gene ID
UniProt
View information about Podocalyxin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 08/21/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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