Purified anti-human CD352 (NTB-A) Antibody

Pricing & Availability
Clone
NT-7 (See other available formats)
Workshop
HCDM listed
Other Names
SLAMF6, LY108, TCOM, SF2000, NTBA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NT-7_Pure_082306
Human peripheral blood lymphocytes stained with purified NT-7, followed by anti-mouse IgG FITC
  • NT-7_Pure_082306
    Human peripheral blood lymphocytes stained with purified NT-7, followed by anti-mouse IgG FITC
Cat # Size Price Quantity Avail. Save
317202 100 µg 84€
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Description

NTBA (NK-, T-, and B-cell Antigen) is a 60 kD type I transmembrane glycoprotein. It is a member of Ig superfamily belonging to CD2/CD150 subfamily, also known as SLAMF6 (SLAM family member 6), LY108, TCOM, or SF2000. This antigen is expressed on NK cells, T cells (upregulated upon activation), and B cells. NTBA is its own ligand. The homophilic interaction of NTBA is involved in the induction of NK cytotoxicity, CD28 independent T cell costimulation, triggering Th1 cytokines production, and regulation of B cell tolerance. It may play a role in the regulation of autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant extracellular domain of NTB-A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Stim - Reported in the Literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: stimulation of NTB-A in a redirected lysis assay and blocking of NTB-A function3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 317204). Co-stimulation of cells with CD3 antibodies and clone NT-7 has been shown to induce activation rather than blocking function of these cells3.

Application References
  1. Messmer B, et al. 2006. J. Immunol. 176:4646. (FC)
  2. Ward J, et al. 2007. Blood 110:1207. (FC) PubMed
  3. Uzana R, et al. 2012. J. Immunol. 188:632. (Block) PubMed
  4. Baglaenko Y, et al. 2017. J. Immunol. 198: 3949. (Stim)
Product Citations
  1. Ward J, et al. 2007. Blood. 110:1207. PubMed
  2. Uzana R, et al. 2012. J Immunol. 188:632. PubMed
Publication Library
RRID
AB_571931 (BioLegend Cat. No. 317202)

Antigen Details

Structure
Type I transmembrane tyrosine-phosphorylated glycoprotein, CD2/CD150 subfamily, 60 kD
Distribution

NK cells, T cells, B cells

Function
CD28 independent T cell costimulation, induction of NK cell cytotoxicity, regulation of B cell tolerance
Ligand/Receptor
NTBA
Cell Type
NK cells, T cells, B cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Bottino C, et al. 2001. J. Exp. Med. 194:235.
2. Valdez PA, et al. 2004. J. Bio. Chem. 279:18662.
3. Flaig RM, et al. 2004. J. Immunol. 172:6524.
4. Falco M, et al. 2004. Eur. J. Immunol. 34:1663.
5. Kumar KR, et al. 2006. Science 312:1665.

Gene ID
114836 View all products for this Gene ID
UniProt
View information about CD352 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05/03/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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