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- Clone
- S7.7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- QA79 membrane protein, Adhesion inhibitory receptor molecule 1, p75/AIRM1, D-siglec
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cy5.5 and purified S7.7 conjugated to PE -
Human peripheral blood monocytes stained with purified S7.7 conjugated to PE
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 347702 | 100 µg | 179€ | ||||
Siglec-7 is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs), also known as p75/AIRM1. It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Siglec-7 3T3 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP, Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: Blocking2.
- Application References
-
- Nicoll G, et al. 1999. J. Biol Chem. 274:34089.
- Hudak J, et al. 2014. Nat. Chem Biol. 10:69–75.
- RRID
-
AB_2189411 (BioLegend Cat. No. 347702)
Antigen Details
- Structure
- Type I transmembrane protein, Siglec family member, 75 kD
- Distribution
-
Monocytes, NK cells
- Function
- Inhibitory receptor of NK cells
- Interaction
- After tyrosine phosphorylation interacts with PTPN6 and SHP-1
- Ligand/Receptor
- Sialylated glycans (with α,8 disialyl and α2,6 sialyl residues)
- Bioactivity
- Inhibition of signal transduction by recruiting cytoplasmic phosphatases
- Cell Type
- Monocytes, NK cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Siglec Molecules
- Antigen References
-
1. Avril T, et al. 2006. Infect Immun. 74:4133.
2. Avril T, et al. 2006. J. Leukoc. Biol. 80:787.
3. Orr SJ, et al. 2007. J. Biol Chem. 282:3418.
4. Brunetta E, et al. 2009. Blood 114:3822. - Gene ID
- 27036 View all products for this Gene ID
- UniProt
- View information about CD328 on UniProt.org
Related FAQs
Other Formats
View All CD328 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD328 (Siglec-7) | S7.7 | FC,IP,Block |
| PE anti-human CD328 (Siglec-7) | S7.7 | FC |
| APC anti-human CD328 (Siglec-7) | S7.7 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD328 (Siglec-7)
Human peripheral blood lymphocytes stained with anti-human C... 
Human peripheral blood monocytes stained with purified S7.7 ... -
PE anti-human CD328 (Siglec-7)
Human peripheral blood lymphocytes stained with anti-human C...
Human peripheral blood monocytes stained with S7.7 PE -
APC anti-human CD328 (Siglec-7)
Human peripheral blood lymphocytes were stained with CD328 (...
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