- 7E7.H12 (See other available formats)
- Regulatory Status
- Other Names
- Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Covance Catalog# MMS-5084
- Mouse IgG1
- Ave. Rating
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- Product Citations
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Chromosomal DNA wraps around histone proteins located in the nucleus of the cell. Histone H3 is one of the five main histone proteins. Featuring a main globular domain and a long N-terminal tail, H3 forms nucleosomes with the 'beads on a string' structure. Histone proteins are highly post-translationally modified and Histone H3 is the most extensively modified. The term "Histone H3" does not distinguish between sequence variants or modification state - including three sequence variants H3.1, H3.2, and H3.3. Histone H3 is important in the field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Histone H3 lysine 9 (H3K9) is a major site of chromatin regulation. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids as well as phosphorylation events. Di- and Tri-methylation of Lysine 9 are associated with repression and heterochromatin, while mono-methylation of K4 is associated with active genes and has been associated with gene silencing and the formation of relatively inactive regions of DNA known as heterochromatin. When H3K9 is acetylated, its neutral charge cannot bind to DNA, resulting in greater access of transcriptional enzymes to DNA. When H3K9 is unmodified, less transcription occurs. The greater the methylation on H3K9, the greater the transcriptional repression.Product Details
- Human, Mouse (and Rat by sequence identity)
- Antibody Type
- Host Species
- This monoclonal antibody was raised against a synthetic peptide from human histone H3, mono-methylated at Lysine-9.
- Phosphate-buffered solution.
- The antibody was purified by affinity chromatography.
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100 - 1000*
• IHC: 1:100
Tissue: Frozen sections
Primary Incubation: 24 hours at 4°C
- Application Notes
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
* Expected MW: 17 kD
WB Positive control: HeLa cell lysate
IHC Positive control tissue: Normal mouse brain
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Product Citations
AB_2564878 (BioLegend Cat. No. 824201)
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