Purified anti-GAPDH Antibody

Pricing & Availability
Clone
W17079A (See other available formats)
Other Names
glyceraldehyde-3-phosphate dehydrogenase
Isotype
Rat IgG2a, κ
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Product Citations
publications
W17079A_PURE_GAPDH_Antibody_1_WB_042718_updated
Whole cell lysates (15 µg protein) from K562, PC3, HeLa, Molt-4, NTRA-2, NIH3T3, and UMR-106 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) Purified anti-GAPDH Antibody, clone W17079A. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405, upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17079A_PURE_GAPDH_Antibody_1_WB_042718_updated
    Whole cell lysates (15 µg protein) from K562, PC3, HeLa, Molt-4, NTRA-2, NIH3T3, and UMR-106 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) Purified anti-GAPDH Antibody, clone W17079A. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405, upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17079A_PURE_GAPDH_Antibody_2_ICC_042418
    HeLa cells were fixed with cold methanol for 15 minutes and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) 1:500 (1 µg/ml) purified rat IgG2a, κ isotype control antibody (Cat. No. 400502) or 1:500 (1 µg/ml, B), 1:2000 (0.25 µg/ml, C) and 1:5000 (0.1 µg/ml, D) anti-GAPDH antibody (clone W17079A) overnight at 4°C followed by Alexa Fluor® 488 (green) conjugated goat anti-rat IgG (Cat. No. 405418) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
607901 25 µg 68€
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607902 100 µg 172€
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Description

GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein (161-335 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.0375-0.5 µg per ml (1:1000-1:16000 dilution). For immunocytochemistry, a concentration range of 0.25-1.0 μg/ml (1:500-1:2000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone weakly reacts with rat GAPDH.

Product Citations
  1. Langer S, et al. 2019. Elife. 8:. PubMed
Publication Library
RRID
AB_2734502 (BioLegend Cat. No. 607901)
AB_2734503 (BioLegend Cat. No. 607902)

Antigen Details

Structure
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution

Ubiquitously expressed, locate in cytoplasm and perinuclear region.

Function
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Interaction
Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
Biology Area
Cell Biology, Western Blot Controls
Antigen References
  1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
  2. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460.
  3. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527.
  4. Zheng L, et al. 2003. Cell. 114:255.
  5. Wang Q, et al. 2005. FASEB J. 19:869.
  6. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
Gene ID
5590 View all products for this Gene ID
UniProt
View information about GAPDH on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/27/2018

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