Purified anti-E. coli RNA Sigma S Antibody

Pricing & Availability
Clone
1RS1 (See other available formats)
Regulatory Status
RUO
Other Names
Sigma 38, rpoS, appR, katF, nur, otsX, sigS, RNA Polymerase
Isotype
Mouse IgG1
Ave. Rating
Submit a Review
Product Citations
publications
1RS1_PURE_Ecoli-RNA-SigmaS_Antibody_1_030320
Whole cell extracts (15 µg protein) from E. coli DH5α cells grown to logarithmic (log) or stationary (stat) phase were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-E. coli RNA Sigma S antibody (clone 1RS1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Ponceau S Red was used to confirm equal protein loading. Lane M: Molecular weight marker.
  • 1RS1_PURE_Ecoli-RNA-SigmaS_Antibody_1_030320
    Whole cell extracts (15 µg protein) from E. coli DH5α cells grown to logarithmic (log) or stationary (stat) phase were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-E. coli RNA Sigma S antibody (clone 1RS1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Ponceau S Red was used to confirm equal protein loading. Lane M: Molecular weight marker.
Cat # Size Price Quantity Check Availability Save
663705 25 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
663706 100 µg 190€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Sigma transcription factor controlling the regulation of genes is required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon, and other genes by modifying the affinity of RNA polymerase for the promoters.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli, Klebsiella, Salmonella, Serratia, Bordetella
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Previously item# W0009 from Neoclone.

Clone 1RS1 does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthobacter, or Borrelia. It recognizes epitope 33-256.

Additional reported applications (for the relevant formats) include: functional assay.

Application References
  1. Nguyen LH, et al. 1993. Biochemistry 32:11112.
RRID
AB_2832841 (BioLegend Cat. No. 663705)
AB_2832842 (BioLegend Cat. No. 663706)

Antigen Details

Structure
Sigma S (σS) is a 38 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947210 View all products for this Gene ID
UniProt
View information about Sigma S on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/03/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Anti-E. coli RNA Sigma S

  • Purified anti-E. coli RNA Sigma S

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account