Purified anti-E. coli RNA Sigma 32 Antibody

Pricing & Availability
Clone
3RH3 (See other available formats)
Regulatory Status
RUO
Other Names
SigmaH, rpoH, fam, hin, htpR, Heat shock regulatory protein F33.4, RNA Polymerase
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
3RH3_purified_E-coli-32_Antibody_1_100818_updated
Western blot analysis of 15 µg cell lysates from DH5α cells. Samples were incubated at 37°C for 0, 5, 10, 15, and 40 minutes and then resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 2 µg/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • 3RH3_purified_E-coli-32_Antibody_1_100818_updated
    Western blot analysis of 15 µg cell lysates from DH5α cells. Samples were incubated at 37°C for 0, 5, 10, 15, and 40 minutes and then resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 2 µg/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • 3RH3_purified_E-coli-32_Antibody_2_100818_updated
    Western blot analysis of 15 µg cell lysates from DH5α and BL21 cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 5 µg/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
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663404 25 µg 76€
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663405 100 µg 190€
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Description

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 3RH3 recognizes epitope 1-25.

Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.

Application References
  1. Lesley SA, et al. 1987. J. Biol. Chem. 262:5404.
RRID
AB_2750247 (BioLegend Cat. No. 663404)
AB_2750248 (BioLegend Cat. No. 663405)

Antigen Details

Structure
Sigma 32 (σ32) is a 32 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947970 View all products for this Gene ID
UniProt
View information about Sigma 32 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/01/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-E. coli RNA Sigma 32

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