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Purified anti-Dopamine D3 receptor Antibody (Previously Covance catalog# MMS-5264)

Pricing & Availability
Clone
N331/19 (See other available formats)
Regulatory Status
RUO
Other Names
DRD3, D3DR, ETM1, FET1, D(3) dopamine receptor, essential tremor 1
Previously
Covance Catalog# MMS-5264
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
N331slas19_Purified_Dopamine-D3-receptor_Antibody_1_041618
ICC staining of purified anti-Dopamine D3 receptor antibody (clone N331/19) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 5 µg/ml of Alexa Fluor® 488 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective.
  • N331slas19_Purified_Dopamine-D3-receptor_Antibody_1_041618
    ICC staining of purified anti-Dopamine D3 receptor antibody (clone N331/19) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 5 µg/ml of Alexa Fluor® 488 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective.
  • N331slas19_Purified_Dopamine-D3-receptor_Antibody_2_041618
    ICC staining of purified anti-Dopamine D3 receptor antibody (clone N331/19) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
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827501 100 µL 132€
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Description

This is the D3 subtype of the five (D1-D5) dopamine receptors. D3 subtype receptor activity is mediated by inhibitory G proteins. This receptor is localized to the limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 223-322 (third intracellular loop) of human Dopamine D3 receptor.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ICC - Quality tested
IHC-P, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration of 10.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunocytochemistry (ICC), formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P), and Western blotting (WB).

Application References
  1. Cote SR and Kuzhikandathil EV, et al. 2015. Neurosci. Lett. 585:33. (WB, IHC)
  2. Ikeda E, et al. 2013. Mol. Pharmacol. 83:959. (WB)
Product Citations
  1. van Nie L, et al. 2020. Sci Rep. 10:11928. PubMed
RRID
AB_2564912 (BioLegend Cat. No. 827501)

Antigen Details

Cell Type
Dopaminergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Synaptic Biology
Molecular Family
GPCR, Postsynaptic proteins
Gene ID
1814 View all products for this Gene ID
UniProt
View information about Dopamine D3 receptor on UniProt.org
Go To Top Version: 4    Revision Date: 06/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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