Purified anti-Cre Recombinase Antibody (Previously Covance catalog# PRB-106P)

Pricing & Availability
Clone
Poly9080 (See other available formats)
Other Names
Cyclization recombinase
Previously
Covance Catalog# PRB-106P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly9080_Pure_Cre-Recombinase_Antibody_1_091420
Total cell lysates (15 µg total protein) from untransfected (-) or Cre recombinase-transfected (+) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:10000 dilution) of purified anti-Cre recombinase antibody (Poly9080) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker.
  • Poly9080_Pure_Cre-Recombinase_Antibody_1_091420
    Total cell lysates (15 µg total protein) from untransfected (-) or Cre recombinase-transfected (+) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:10000 dilution) of purified anti-Cre recombinase antibody (Poly9080) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker.
Cat # Size Price Quantity Avail. Save
908002 25 µL 60€
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908001 200 µL 244€
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Description

Cre-recombinase is a 38 kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. This polyclonal antibody was raised against a full-length Cre Recombinase recombinant protein and was subsequently purified using Protein A. It can be used to determine the success of gene manipulations in transgenic animals.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Bacteriophage Cre Recombinase
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution + 1% BSA and 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
Store between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.025 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Leiton CV, et al. 2018. Hypoxia (Auckl). 6:35. PubMed
  2. Choe Y, et al. 2018. Brain Plast. 4:169. PubMed
  3. Costa TDF, et al. 2019. Nat Commun. 10:3589. PubMed
  4. Hoffmann HM, et al. 2019. Neuroendocrinology. 108:328. PubMed
  5. Bäck S, et al. 2019. Neuron. 102:105. PubMed
  6. Karen J Tonsfeldt et al. 2019. Journal of the Endocrine Society. 3(4):716-733 . PubMed
  7. Holt LJ, et al. 2018. Endocrinology. 159:1339. PubMed
  8. Dudok J, et al. 2016. Neurosci Res. S0168-0102(16)00002-X. PubMed
  9. Benedykcinska A, et al. 2016. Dis Model Mech. 9: 211 - 220. PubMed
  10. Anderson T, et al. 2016. Nature. 10.1038/nature18944. PubMed
  11. Heuer A, et al. 2016. Exp Neurol. 282:78-85. PubMed
  12. Dvorakova M, et al. 2016. Sci Rep. 6:38253. PubMed
  13. Twining R, et al. 2017. Nat Neurosci. 20:459-469. PubMed
RRID
AB_2801251 (BioLegend Cat. No. 908002)
AB_2565079 (BioLegend Cat. No. 908001)

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Go To Top Version: 4    Revision Date: 09/14/2020

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This data display is provided for general comparisons between formats.
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