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Purified anti-Cre Recombinase Antibody (Previously Covance catalog# PRB-106P)

Pricing & Availability
Clone
Poly9080 (See other available formats)
Regulatory Status
RUO
Other Names
Cyclization recombinase
Previously
Covance Catalog# PRB-106P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly9080_Pure_Cre-Recombinase_Antibody_1_091420
Total cell lysates (15 µg total protein) from untransfected (-) or Cre recombinase-transfected (+) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:10000 dilution) of purified anti-Cre recombinase antibody (Poly9080) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker.
  • Poly9080_Pure_Cre-Recombinase_Antibody_1_091420
    Total cell lysates (15 µg total protein) from untransfected (-) or Cre recombinase-transfected (+) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:10000 dilution) of purified anti-Cre recombinase antibody (Poly9080) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker.
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908002 25 µL 72€
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908001 200 µL 287€
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Description

Cre-recombinase is a 38 kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. This polyclonal antibody was raised against a full-length Cre Recombinase recombinant protein and was subsequently purified using Protein A. It can be used to determine the success of gene manipulations in transgenic animals.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Bacteriophage Cre Recombinase
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution + 1% BSA and 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
Store between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.025 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Dvorakova M, et al. 2016. Sci Rep. 6:38253. PubMed
  2. Bäck S, et al. 2019. Neuron. 102:105. PubMed
  3. Anderson T, et al. 2016. Nature. 10.1038/nature18944. PubMed
  4. Karen J Tonsfeldt et al. 2019. Journal of the Endocrine Society. 3(4):716-733 . PubMed
  5. Bohuslavova R, et al. 2021. Int J Mol Sci. 22:. PubMed
  6. Holt LJ, et al. 2018. Endocrinology. 159:1339. PubMed
  7. Twining R, et al. 2017. Nat Neurosci. 20:459-469. PubMed
  8. Dvorakova M, et al. 2020. Dev Biol. 457:43:00. PubMed
  9. Leiton CV, et al. 2018. Hypoxia (Auckl). 6:35. PubMed
  10. Choe Y, et al. 2018. Brain Plast. 4:169. PubMed
  11. Filova I, et al. 2020. Mol Neurobiol. 57:5307. PubMed
  12. Jecrois ES, et al. 2021. Developmental Cell. :. PubMed
  13. Costa TDF, et al. 2019. Nat Commun. 10:3589. PubMed
  14. Dudok J, et al. 2016. Neurosci Res. S0168-0102(16)00002-X. PubMed
  15. Heuer A, et al. 2016. Exp Neurol. 282:78-85. PubMed
  16. Benedykcinska A, et al. 2016. Dis Model Mech. 9: 211 - 220. PubMed
  17. Hoffmann HM, et al. 2019. Neuroendocrinology. 108:328. PubMed
  18. Chung EJ, et al. 2021. Int J Radiat Oncol Biol Phys. 110:526. PubMed
RRID
AB_2801251 (BioLegend Cat. No. 908002)
AB_2565079 (BioLegend Cat. No. 908001)

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Go To Top Version: 4    Revision Date: 09/14/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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  • Purified anti-Cre Recombinase

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