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Purified anti-CDK5 Antibody

Pricing & Availability
Clone
P84E4 (See other available formats)
Regulatory Status
RUO
Other Names
Cyclin-Dependent Kinase 5, Serine/Threonine-Protein Kinase PSSALRE, Tau Protein Kinase II Catalytic Subunit, Cell Division Protein Kinase 5, TPKII Catalytic Subunit, Cyclin-Dependent-Like Kinase 5, CDKN5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P84E4_PURE_CDK5_Antibody_WB_070618
Total cell lysates (15 µg protein) from SH-SY5Y (lane 1), HeLa (lane 2), HepG2 (lane 3) and NIH3T3 (lane 4) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-CDK5 antibody (clone P84E4) (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-CDK5 antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower blot). Lane M: Molecular weight ladder.
  • P84E4_PURE_CDK5_Antibody_WB_070618
    Total cell lysates (15 µg protein) from SH-SY5Y (lane 1), HeLa (lane 2), HepG2 (lane 3) and NIH3T3 (lane 4) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-CDK5 antibody (clone P84E4) (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-CDK5 antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower blot). Lane M: Molecular weight ladder.
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684502 100 µg 249€
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Description

Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases known for their roles in the control of cell cycle progression. CDK5 is an atypical member in that it plays important roles in almost all aspects of brain development and function; it regulates multiple players required for axon formation and regeneration. CDK5 functions as a "plastic" kinase that maintains the axon growth ability by enabling efficient cytoskeletal reorganization, enhancing protein translation, reducing protein degradation, and promoting injury-induced gene transcription. Over-activation of CDK5 is associated with many neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Furthermore, CDK5 is reportedly involved in T cell activation and plays an important role in development of autoimmune disorders such as multiple sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment (aa 120-292) of human CDK5 expressed in E.coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2572168 (BioLegend Cat. No. 684502)

Antigen Details

Structure
292 amino acids with a predicted molecular weight of approximately 33kD.
Distribution

Ubiquitous expression of catalytic subunit in all cells and tissue types, while expression of p35 is restricted mainly to neurons.

Function
CDK5 plays important roles in almost all aspects of brain development and function; it regulates multiple players required for axon formation and regeneration.
Interaction
CDK5R1 (p25), CDK5R1 (p35), CDK5RAP1, CDK5RAP2, CDK5RAP3, CABLES1, ABL1, CABLES1, CABLES2, AATK, GSTP1, HDAC1, beta-catenin/CTNNB1, delta-catenin/CTNND2, APEX1, P53/TP53, EPHA4, NGEF, EPHA4, PTK2/FAK1, CLOCK and HTR6.
Ligand/Receptor
CDK5R1 and CDK5R2.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immunology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers, Protein Synthesis, Signal Transduction, Synaptic Biology, Transcription Factors
Molecular Family
Postsynaptic proteins, Protein Kinases/Phosphatase
Antigen References

1. Wilkaniec A, et al. 2015. J. Neurochem. 10.1111/jnc.13365.
2. Liu SL, et al. 2015. Mol. Neurobiol.
3. Lai KO, et al. 2015. Mini. Rev. Med. Chem. 15:390.
4. Ye T, et al. 2012. Int. Rev. Neurobiol. 105:91.
5. Cheung ZH, et al. 2012. Trends Cell Biol. 22:169.
6. Lalioti V, et al. 2010. Cell Cycle 9:284.
7. Slevin M, et al. 2009. Curr. Opin. Pharmacol. 9:119.
8. Dhavan R, et al. 2001. Nat. Rev. Mol. Cell. Biol. 2:749.
9. Pareek TK, et al. 2010. J. Exp. Med. 207:2507.

Gene ID
1020 View all products for this Gene ID
UniProt
View information about CDK5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/06/2018

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