- W16197A (See other available formats)
- Other Names
- ACTB, Actin, cytoplasmic 1, Beta-actin
- Rat IgG2a, κ
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- Product Citations
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β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).Product Details
- Human, Mouse, Rat, Zebra Fish, Drosophila
- Antibody Type
- Host Species
- Human ACTB peptide (1-13 a.a.).
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.1 µg/ml in human, mouse, rat and zebrafish and 0.1 - 0.5 µg/ml in Drosophila. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Reactivity to Drosophila was only validated with the purified format.
AB_2721350 (BioLegend Cat. No. 664801)
AB_2721349 (BioLegend Cat. No. 664802)
- 375 amino acids with a predicted molecular weight of 42 kD.
- Actins are highly conserved proteins that are involved in cell motility.
- Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers, Western Blot Controls
- Molecular Family
- Antigen References
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
- Gene ID
- 60 View all products for this Gene ID
- View information about beta-actin on UniProt.org
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