Purified anti-AU5 Epitope Tag Antibody (Previously Covance catalog# MMS-135P)

Pricing & Availability
AU5 (See other available formats)
Regulatory Status
Other Names
AU5 epitope tag, TDFYLK epitope tag, TDFYLK tag
Covance Catalog# MMS-135P
Mouse IgG1
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Product Citations
Cat # Size Price Quantity Check Availability Save
902007 25 µL 72€
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902002 500 µL 445€
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The AU5 tag, which consists of the peptide sequence TDFYLK, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and other immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
The AU5 monoclonal antibody was raised against an SDS-disrupted BPV-1 preparation and mapped to the six amino acid epitope TDFYLK.
Phosphate-buffered solution + 0.03% Thimerosal.
The antibody was purified by affinity chromatography.
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.

WB, ICC, IP, Purification

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:500*
ICC: 1:500
IP: 1:150

Application Notes

This antibody is effective in immunofluorescence (IF), immunoblotting, and immunoprecipitation (IP) of tagged proteins.

This clone was specifically designed for use in epitope tagging. Because of its small size, clone AU5 is unlikely to alter the activity of the cloned sequence. This antibody was purified using protein-G chromatography.

Application References
  1. Burke TW, et al. 2001. J Biol Chem. 276:15397.
  2. Marinissen MJ, et al. 2001. Genes Dev. 15:535.
  3. Lim PS, et al. 1990. J Infect Dis. 162:1263.
Product Citations
  1. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
AB_2734668 (BioLegend Cat. No. 902007)
AB_2565015 (BioLegend Cat. No. 902002)

Antigen Details

Biology Area
Cell Biology
Gene ID
View information about AU5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 01/15/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Anti-AU5 Epitope Tag Affinity Matrix

  • Purified anti-AU5 Epitope Tag


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