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PerCP/Cyanine5.5 anti-mouse CD19 Antibody

Pricing & Availability
Clone
1D3/CD19 (See other available formats)
Regulatory Status
RUO
Other Names
B4, CVID3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1D3slashCD19_PerCPCy5.5_CD19_Antibody_090216
C57BL/6 mouse splenocytes were stained with CD19 (clone 1D3/CD19) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 1D3slashCD19_PerCPCy5.5_CD19_Antibody_090216
    C57BL/6 mouse splenocytes were stained with CD19 (clone 1D3/CD19) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
152405 25 µg 81€
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152406 100 µg 268€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Transfected k562 cells expressing the extracellular domain of murine CD19.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
  2. Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
Product Citations
  1. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  2. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  3. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  4. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  5. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  6. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  7. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  8. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  9. Dai G, et al. 2021. mSphere. 6: . PubMed
  10. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  11. Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed
  12. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  13. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  14. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  15. Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed
  16. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  17. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  18. Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed
RRID
AB_2629814 (BioLegend Cat. No. 152405)
AB_2629815 (BioLegend Cat. No. 152406)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD.
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells.

Function
Modulates B cell activation and differentiation.
Ligand/Receptor
CD21, CD81, Leu-13.
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 09/02/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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