PE Rat IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2758 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
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Product Citations
publications
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400507 25 µg 32.00€
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400508 100 µg 64.00€
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Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
KLH
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References
  1. Nishimoto H, et al. 2005. Blood 106:4241.
  2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
  3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations
  1. Zape J, et al. 2017. Cell Cycle. 10.1080/15384101.2017.1361569. PubMed
  2. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
  3. Li ZG, et al. 2018. Mil Med Res. 5:24. PubMed
  4. Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed
  5. Arya SB, et al. 2018. J Biol Chem. 293:9892. PubMed
  6. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  7. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  8. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  9. Grisanti LA, et al. 2019. JCI Insight. 5. PubMed
  10. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  11. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  12. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  13. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  14. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  15. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  16. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  17. Harada Y, et al. 2019. Pain. 160:2050. PubMed
  18. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  19. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
  20. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
  21. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
  22. Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed
  23. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  24. Kubiniok P, et al. 2019. Mol Cell Proteomics. 18:408. PubMed
  25. Cardot-Ruffino V, et al. 2020. Sci Rep. 10:3880. PubMed
  26. Chou PR, et al. 2020. Int J Med Sci. 17:354. PubMed
  27. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  28. Rajashekara G, et al. 2008. J Bacteriol. 190:6243. PubMed
  29. Schmidt N, et al. 2011. J Immunol. 186:5873. PubMed
  30. Toda S, et al. 2014. Blood. 123:3963. PubMed
  31. Adamopoulos I, et al. 2015. Ann Rheum Dis. 74:1284. PubMed
  32. Madera L, et al. 2015. PLoS One. 10: 0133385. PubMed
  33. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
  34. Sockolosky J, et al. 2016. Proc Natl Acad Sci U S A. 113: 2646 - 2654. PubMed
  35. Asson-Batres M, et al. 2016. Am J Physiol Heart Circ Physiol. 310: 1773 - 1789. PubMed
  36. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  37. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  38. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  39. Hauptmann M, et al. 2017. Infect Immun . 10.1128/IAI.00156-17. PubMed
  40. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
  41. Takayanagi Y, et al. 2020. J Clin Med. 9:00. PubMed
  42. Kovacs L, et al. 2020. PLoS One. 15:e0240455. PubMed
  43. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  44. Cardot-Ruffino V, et al. 2020. Genesis. 58:e23359. PubMed
  45. Nott A, et al. 2021. Nature Protocols. 16(3):1629-1646. PubMed
  46. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  47. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  48. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  49. Barkaway A, et al. 2021. Immunity. . PubMed
  50. Yang Z, et al. 2020. J Invest Dermatol. 544:140. PubMed
  51. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
RRID
AB_326530 (BioLegend Cat. No. 400508)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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