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PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_PECy7_091207
C57BL/6 mouse splenocytes stained with D7 PE/Cyanine7
  • D7_PECy7_091207
    C57BL/6 mouse splenocytes stained with D7 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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108113 25 µg 82€
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108114 100 µg 253€
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  2. Wu J, et al. 2022. Stem Cells Int. 2022:4249843. PubMed
  3. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  4. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  5. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  6. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  7. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  8. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  9. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  10. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  11. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  12. DellOrso S, et al. 2021. STAR Protocols. 2(2):100451. PubMed
  13. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  14. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  15. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  16. Campanario S, et al. 2021. Front Cell Dev Biol. 8:620409. PubMed
  17. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  18. Solanas G, et al. 2017. Cell. 170:678. PubMed
  19. Gross ETE, et al. 2018. Oncoimmunology. 8:e1404212. PubMed
  20. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  21. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  22. Arai F, et al. 2020. Cell Systems. 11(6):640-652.e5. PubMed
  23. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  24. Ankawa R, et al. 2021. Developmental Cell. . PubMed
  25. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  26. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  27. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  28. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  29. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  30. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  31. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  32. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  33. Dong S, et al. 2021. Nature. 591:117. PubMed
  34. Smith E, et al. 2009. J Immunol. 183:5685. PubMed
  35. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  36. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  37. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  38. Duan Y, et al. 2018. Stem Cells. 36:1430. PubMed
  39. Cho K, et al. 2021. iScience. 24:103117. PubMed
  40. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
  41. García-Prat L, Muñoz-Cánoves P, Martínez-Vicente 2017. Muscle Stem Cells. 10.1007/978-1-4939-6771-1_14. PubMed
  42. Zaro BW, et al. 2020. eLife. 9:00. PubMed
  43. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  44. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  45. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  46. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  47. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  48. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  49. Gough D, et al. 2014. Blood. 124:2252. PubMed
  50. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  51. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  52. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  53. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  54. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  55. Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
  56. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  57. Kang M, et al. 2021. Front Cell Dev Biol. 8:596622. PubMed
  58. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  59. Azazmeh N, et al. 2020. Nat Commun. 2.340972222. PubMed
  60. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  61. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  62. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  63. Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed
  64. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  65. Liu F, et al. 2021. Cell Reports. 35(10):109225. PubMed
  66. Jin Y, et al. 2020. PeerJ. 8:e10374. PubMed
  67. Yosef R, et al. 2016. Nat Commun. 7: 11190. PubMed
  68. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  69. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  70. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  71. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
  72. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  73. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  74. Chavez JS, et al. 2022. Cells. 11:. PubMed
  75. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  76. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  77. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  78. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  79. Del Barco Barrantes I, et al. 2018. Stem Cell Reports. 10:257. PubMed
  80. Zhang B, et al. 2021. Nature. 599:471. PubMed
  81. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  82. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  83. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  84. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  85. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  86. Youssif C, et al. 2018. EMBO Mol Med. 10:e8403. PubMed
  87. Hacein-Bey-Abina S, et al. 2020. Exp Hematol. 88:15. PubMed
  88. Man N, et al. 2021. JCI Insight. 6:. PubMed
RRID
AB_493597 (BioLegend Cat. No. 108113)
AB_493596 (BioLegend Cat. No. 108114)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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