PE/Cyanine7 anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
IM7_PECy7_011108
C57BL/6 mouse splenocytes stained with IM7 PE/Cyanine7
  • IM7_PECy7_011108
    C57BL/6 mouse splenocytes stained with IM7 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
103029 25 µg 68€
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103030 100 µg 156€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Chulkina M, et al. 2017. Eur J Pharmacol. 10.1016/j.ejphar.2017.06.033. PubMed
  2. Limbach K, et al. 2017. Malar J. 10.1186/s12936-017-1911-z. PubMed
  3. Belogortseva N, et al. 2017. PLoS One.. 10.1371/journal.pone.0182825. PubMed
  4. LaMothe RA et al. 2018. Front Immunol. 0.570138889 . PubMed
  5. Wang D, et al. 2018. Immunity. 48:659. PubMed
  6. Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed
  7. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  8. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  9. Han Y, et al. 2019. Am J Cancer Res. 9:800. PubMed
  10. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  11. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  12. Ise W, et al. 2018. Immunity. 48:702. PubMed
  13. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  14. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  15. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  16. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  17. Pal D et al. 2017. eLife. 6 pii: e21615. PubMed
  18. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  19. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  20. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  21. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  22. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  23. Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed
  24. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  25. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  26. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  27. Yomtoubian S, et al. 2020. Cell Reports. 30(3):755-770.e6.. PubMed
  28. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  29. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  30. Ueda R, et al. 2009. Clin Cancer Res. 5.215972222. PubMed
  31. Almeida D, et al. 2010. J Immunol. 185:1927. PubMed
  32. Gondek D, et al. 2012. J Immunol. 189:2441. PubMed
  33. Bolinger B, et al. 2013. J Immunol. 190:4162. PubMed
  34. Kitagawa K, et al. 2014. Mol Cell Biol. 34:2732. PubMed
  35. Kersh A, et al. 2014. J Immunol. 193:4429. PubMed
  36. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  37. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  38. Aihara E, et al. 2015. Sci Rep. 5:17185. PubMed
  39. Vishnoi M, et al. 2015. Sci Rep. 5: 17533. PubMed
  40. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  41. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  42. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  43. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
RRID
AB_830786 (BioLegend Cat. No. 103029)
AB_830787 (BioLegend Cat. No. 103030)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/11/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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