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PE anti-Th-POK (ZFP-67) Antibody

Pricing & Availability
Clone
11H11A14 (See other available formats)
Regulatory Status
RUO
Other Names
T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 7B (ZBTB7B), Krueppel-related zinc finger protein (cKrox), Zinc finger protein 67 homolog (ZFP-67)
Isotype
Mouse IgG1, κ
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Product Citations
publications
11H11A14_PE_Th-pok_Antibody_1_ICFC_090815
Human peripheral blood lymphocytes were surface stained with CD4 Brilliant Violet 421™ and CD8 Alexa Fluor® 647, and then were treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with Th-POK (clone 11H11A14) PE (filled histogram: CD4-positive cells, open histogram with solid line: CD8-positive cells) or mouse IgG1, κ PE isotype control (open histogram with dotted line: CD4-positive cells, open histogram with dashed line: CD8-positive cells).
  • 11H11A14_PE_Th-pok_Antibody_1_ICFC_090815
    Human peripheral blood lymphocytes were surface stained with CD4 Brilliant Violet 421™ and CD8 Alexa Fluor® 647, and then were treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with Th-POK (clone 11H11A14) PE (filled histogram: CD4-positive cells, open histogram with solid line: CD8-positive cells) or mouse IgG1, κ PE isotype control (open histogram with dotted line: CD4-positive cells, open histogram with dashed line: CD8-positive cells).
  • 11H11A14_PE_Th-pok_Antibody_2_ICFC_090815
    C57BL/6 thymocytes were surface stained with CD8 APC/Cy7 and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with Th-POK (clone 11H11A14) PE.
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656403 25 tests 113€
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656404 100 tests 288€
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Description

Th-POK is a member of the BTB-POZ domain-containing zinc finger transcription factor family. It regulates gene expression during intrathymic T cell differentiation. A single autosomal recessive mutation of the Th-POK gene results in the absence of mature CD4+ helper T cells, called helper-deficient (HD) phenotype. In Th-POK deficient mice, MHC class II restricted thymocytes are redirected to the CD8+ T cell lineage. On the contrary, forced expression of Th-POK redirects MHC class I restricted thymocytes to the CD4+ T cell lineage. Inactivation of Th-POK in mature CD4+ T cells results in induction of the genes preferentially expressed in CD8+ T cells, such as CD8, granzyme B, and IFN-γ. These findings suggest that Th-POK is essential in promoting commitment of immature T cells to the CD4 lineage and maintaining the characteristics of mature CD4+ T helper cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Th-POK recombinant protein (197-345 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone displays a similar affinity to BioLegend clone W15147A by western blot. However, this clone does not recognize mouse TH-POK.

RRID
AB_2563008 (BioLegend Cat. No. 656403)
AB_2563009 (BioLegend Cat. No. 656404)

Antigen Details

Structure
539 amino acids, predicted molecular weight of 58 kD; contains four C2H2-type zinc finger regions that are responsible for DNA binding; contains a BTB-POZ domain which is involved in homodimerization and association with other factors
Distribution

Nucleus

Function
Transcription factor that regulates CD4 lineage commitment of immature T-cell precursors; acts as a transcriptional repressor of collagen and fibronectin genes
Interaction
Acetyltransferase p300
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
TCRs
Antigen References

1. Kappes DJ. 2010. Immunol. Rev. 238:182.
2. Mariani F, et al. 2013. PLoS One. 8:e54488.
3. Zhang M, et al. 2010. J. Immunol. 185:3960.
4. Egawa T, et al. 2008. Nat. Immunol. 9:1131.
5. Wildt KF, et al. 2007. J. Immunol. 179:4405.
6. Setoguchi R, et al. 2009. J. Immunol. 183:4467.

Gene ID
51043 View all products for this Gene ID
UniProt
View information about Th-POK on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09/08/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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