PE anti-mouse CD289 (TLR9) Antibody

Pricing & Availability
Clone
S18025A (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
S18025A_PE_CD289_Antibody_1_070820.png
C57BL/6 mouse splenocytes were stained with CD317 Pacific Blue™, fixed, permeabilized and intracellularly stained with CD289 (TLR9) (clone S18025A) PE (left) or rat IgG1, κ isotype control PE (right).
  • S18025A_PE_CD289_Antibody_1_070820.png
    C57BL/6 mouse splenocytes were stained with CD317 Pacific Blue™, fixed, permeabilized and intracellularly stained with CD289 (TLR9) (clone S18025A) PE (left) or rat IgG1, κ isotype control PE (right).
  • S18025A_PE_CD289_Antibody_2_070820.png
    C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/Cyanine7, fixed, permeabilized and intracellularly stained with CD289 (TLR9) (clone S18025A) PE (left) or rat IgG1, κ isotype control PE (right).
See PE spectral data
Cat # Size Price Quantity Avail. Save
159103 25 µg 100€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
159104 100 µg 244€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Proprietary
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2876552 (BioLegend Cat. No. 159103)
AB_2876552 (BioLegend Cat. No. 159104)

Antigen Details

Distribution

B cells, Dendritic cells, pDCs

Cell Type
B cells, Dendritic cells
Biology Area
Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References
  1. Onji M, et al. 2013. Nat. Commun. DOI: 10.1038/ncomms2949
  2. Krug A, et al. 2001. Eur J Immunol. 10:3026-37.
Gene ID
81897 View all products for this Gene ID
UniProt
View information about CD289 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/08/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account