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PE anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
281-2_PE_1_101211
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.
  • 281-2_PE_1_101211
    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.
  • 281-2_PE_2_101211
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142503 25 µg 108€
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142504 200 µg 281€
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Application References
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  2. Jash A, et al. 2016. J Immunol. 197: 1159 - 1168. PubMed
  3. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  4. Wang L, et al. 2019. Cancer Lett. 440-441:135. PubMed
  5. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  6. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  7. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  8. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  9. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  10. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  11. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  12. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  13. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  14. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  15. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  16. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  17. Klessing S, et al. 2020. Vaccines (Basel). 8:. PubMed
  18. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  19. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  20. Chen M, et al. 2016. J Immunol . 194: 2607-15. PubMed
  21. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  22. Roy K, et al. 2019. Immunity. 50:616. PubMed
  23. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  24. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  25. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  26. Li H, et al. 2020. Nature. 584:274. PubMed
  27. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  28. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  29. Ribeiro de Almeida C, et al. 2018. Mol Cell. 70:650. PubMed
  30. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  31. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  32. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
  33. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  34. Schager AE, et al. 2018. MBio. 9:02379. PubMed
  35. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  36. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
  37. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  38. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  39. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  40. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  41. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  42. Che N, et al. 2021. Front Immunol. 12:626310. PubMed
  43. Song W, et al. 2022. Immunity. 55:290. PubMed
  44. Steinmetz TD, et al. 2021. Autophagy. 17:2238. PubMed
  45. BJ L, et al. 2017. J Exp Med . 10.1084/jem.20161461. PubMed
RRID
AB_10915989 (BioLegend Cat. No. 142503)
AB_10916119 (BioLegend Cat. No. 142504)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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