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PE anti-human IFN-γ Antibody

Pricing & Availability
Clone
W19227A (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19227A_PE_IFN-gamma_Antibody_080422
PMA+Ionomycin (4 hours) stimulated human PBMCs were surface stained with anti-human CD3 APC then fixed, permeabilized, and intracellularly stained with anti-human IFN-γ (clone W19227A) PE (left), or rat IgG2a, κ PE isotype control (right).
  • W19227A_PE_IFN-gamma_Antibody_080422
    PMA+Ionomycin (4 hours) stimulated human PBMCs were surface stained with anti-human CD3 APC then fixed, permeabilized, and intracellularly stained with anti-human IFN-γ (clone W19227A) PE (left), or rat IgG2a, κ PE isotype control (right).
See PE spectral data
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383303 25 tests 80€
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383304 100 tests 196€
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Description

Human interferon-gamma (IFN-γ, type II interferon) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells in response to antigens and other cytokines. Originally characterized based on anti-viral activities. IFN-γ also exerts tumor anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II, and macrophage activation. This human IFN-γ antibody does not show cross-reactivity with mouse.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2924583 (BioLegend Cat. No. 383303)
AB_2924583 (BioLegend Cat. No. 383304)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Distribution

CD8+ and CD4+ T cells, NK cells

Function
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH 2
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
  2. De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6.
  3. Farrar M, et al. 1993. Annu Rev Immunol. 11:571-611.
  4. Gray P, et al. 1987. Lymphokines. 13:151.
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/04/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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