PE anti-human CD49e Antibody

Pricing & Availability
Clone
NKI-SAM-1 (See other available formats)
Regulatory Status
RUO
Other Names
α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
NKI-Sam-1_PE_021508
Human peripheral blood lymphocytes stained with NKI-Sam-1 PE
  • NKI-Sam-1_PE_021508
    Human peripheral blood lymphocytes stained with NKI-Sam-1 PE
See PE spectral data
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328009 25 tests 88.00€
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328010 100 tests 192.00€
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Description

CD49e is a type I integral membrane glycoprotein, known as α5 integrin, VLA-5 α chain. It forms noncovalent heterodimer with integrin β1 (CD29). CD49e contains two disulfide-linked subunits of 135 kD and 24 kD, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
U937 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of a5 integrin mediated cell adhesion2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 328004).

Application References
  1. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  2. Orecchia A, et al. 2003. J. Cell Sci. 116:3479. (Block)
Product Citations
  1. Frank N, et al. 2005. Cancer Res. 65:4320. PubMed
  2. Ferreira L, et al. 2016. Proc Natl Acad Sci U S A. 113: 5364 - 5369. PubMed
RRID
AB_893368 (BioLegend Cat. No. 328009)
AB_2280539 (BioLegend Cat. No. 328010)

Antigen Details

Structure
Integrin family, associates with integrin ß1 (CD29), 135 kD and 25 kD in reduced condition, 155 kD (unreduced)
Distribution

Thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, some myeloid leukemia cells

Function
Adhesion, regulation of cell survival and apoptosis
Ligand/Receptor
Fibronectin, neural adhesion molecule L1
Cell Type
Endothelial cells, Leukemia, Lymphocytes, Mesenchymal Stem Cells, Osteoclasts, Thymocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1.Tran Van Nhieu GT and Isberg RR. 1991. J. Biol. Chem. 266:24367.
2. Isaji T, et al. 2006.J. Biol. Chem. 281:33258.

Gene ID
3678 View all products for this Gene ID
UniProt
View information about CD49e on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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