PE anti-Biotin Antibody

Pricing & Availability
Clone
1D4-C5 (See other available formats)
Regulatory Status
RUO
Other Names
Vitamin B7, Vitamin H
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1D4-C5_PE_071310
C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), followed by 1D4-C5 PE
  • 1D4-C5_PE_071310
    C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), followed by 1D4-C5 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
409003 25 µg 52.00€
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409004 100 µg 140.00€
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Description

Biotin (vitamin B7 or vitamin H) is essential for cell growth and the metabolization of fat and protein. It is also often used to tag a molecule of interest. The anti-biotin antibody can be used as a second-step reagent for detecting biotinylated targets.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Biotinylated limulus hemocyanin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation and ELISA.

Application References
  1. Uccellini MB, et al. 2008. J. Immunol. 181:5875.
  2. Green NM, et al. 2012. J Biol Chem. 287:39789. PubMed.
Product Citations
  1. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  2. Zou F, et al. 2019. Nat Commun. 10:4109. PubMed
  3. Chan W, et al. 2014. Leukemia. . PubMed
  4. Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
  5. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
  6. Howe EN, et al. 2020. Nat Commun. 2.553472222. PubMed
RRID
AB_10645482 (BioLegend Cat. No. 409003)
AB_10641847 (BioLegend Cat. No. 409004)

Antigen Details

Ligand/Receptor
Avidin, Streptavidin
Gene ID
NA
UniProt
View information about Biotin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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