GoInVivo™ Purified anti-human CD152 (CTLA-4) Antibody

Pricing & Availability
Clone
L3D10 (See other available formats)
Other Names
Cytotoxic T-Lymphocyte Antigen 4
Isotype
Mouse IgG1, κ
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Product Citations
publications
L3D10_GoInVivoPurified_CD152_CTLA-4_Antibody_FC_020316
Anti-human CD152 (CTLA-4) inhibits production of IL-2 by Jurkat cells. When Jurkat cells are stimulated with PHA (2.5 µg/ml) and soluble CD80 (B7.1, 500 ng/ml), the addition of soluble human CTLA-4 competes with CD80 and inhibits the production of IL-2 in a dose dependent manner (diamonds). When a fixed concentration of CTLA-4 is selected and pre-incubated with increasing concentrations of anti-human CTLA-4 (clone L3D10), the production of IL-2 is restored as the antibody blocks the activity of CTLA-4, but not the isotype control (clone MOPC-21).
  • L3D10_GoInVivoPurified_CD152_CTLA-4_Antibody_FC_020316
    Anti-human CD152 (CTLA-4) inhibits production of IL-2 by Jurkat cells. When Jurkat cells are stimulated with PHA (2.5 µg/ml) and soluble CD80 (B7.1, 500 ng/ml), the addition of soluble human CTLA-4 competes with CD80 and inhibits the production of IL-2 in a dose dependent manner (diamonds). When a fixed concentration of CTLA-4 is selected and pre-incubated with increasing concentrations of anti-human CTLA-4 (clone L3D10), the production of IL-2 is restored as the antibody blocks the activity of CTLA-4, but not the isotype control (clone MOPC-21).
Cat # Size Price Quantity Avail. Save
349923 5 mg 560€
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349924 25 mg 1,720€
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349925 50 mg 2,600€
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349915 100 mg 3,880€
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349916 500 mg 13,400€
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349917 1 g 20,800€
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Description

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <1.0 EU/mg of the protein. (<0.1 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

ELISA Detection: The biotinylated L3D10 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified A3.6B10.G1 antibody (Cat. No. 525401) as the capture antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.

Flow Cytometry: The fluorochrome-labeled L3D10 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify CTLA-4-producing cells within mixed cell populations.

Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407 & 437408) are specially developed and recommended.

Additional reported applications (for the relevant formats) include: Blocking of CTLA-4/B7-1 interaction and blocking of CTLA-4-mediated inhibitory function to promote T cell expansion1,2.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).

Application References
  1. May K, et al. 2005. Blood 105:1114. (Block)
  2. Lute K, et al. 2005. Blood 106:3127. (Block)
RRID
AB_2566296 (BioLegend Cat. No. 349923)
AB_2566297 (BioLegend Cat. No. 349924)
AB_2566298 (BioLegend Cat. No. 349925)
AB_2566083 (BioLegend Cat. No. 349915)
AB_2566084 (BioLegend Cat. No. 349916)
AB_2566085 (BioLegend Cat. No. 349917)

Antigen Details

Structure
Ig superfamily, 33 kD
Distribution

Activated T cells and B cells

Function
Negative regulator of T cell activation
Interaction
Antigen presenting cells, such as dendritic cells
Ligand/Receptor
B7-1 (CD80), B7-2 (CD86)
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.

Gene ID
1493 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11/30/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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