Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 400301 and 400302 are available as an alternative.
Description
The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Each lot of this antibody is quality control tested by ChIP assay as negative control. Use at concentrations comparable to those of the specific antibody of interest.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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