Go-ChIP-Grade™ Purified anti-TP63 Antibody

Pricing & Availability
Clone
W15093A (See other available formats)
Other Names
Tumor protein p63, p63, Chronic ulcerative stomatitis protein (CUSP), Keratinocyte transcription factor (KET), Tumor protein p73-like (p73L)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
W15093A_GoChIP_Antibody_1_011118
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HaCaT cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 Antibody (clone W15093A, Cat. No. 687203) or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody (Clone MG2b-57, Cat. No. 401201) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • W15093A_GoChIP_Antibody_1_011118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HaCaT cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 Antibody (clone W15093A, Cat. No. 687203) or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody (Clone MG2b-57, Cat. No. 401201) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • W15093A_GoChIP_Antibody_2_011118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HaCaT cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 Antibody (clone W15093A, Cat. No. 687203) or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody (Clone MG2b-57, Cat. No. 401201) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • W15093A_PURE_TP63_Antibody_1_WB_111617_Updated
    15 µg total protein from NIH3T3 (lane 1), HaCat (lane 2) and 10 µg total protein from 293E overexpressed with TA-p63α (lane 3) and ΔN-p63α (lane 4) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) or 1:106 (0.0005 µg/ml) purified anti-TP63 antibody, clone W15093A (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder. This antibody recognizes more than one isoform of ΔN-p63 which might be detected lower than 75kD.
  • W15093A_PURE_TP63_Antibody_2_IF_052316
    HaCat cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 5.0 µg/ml purified anti-TP63 (clone W15093A) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated anti-mouse IgG antibody (red), Alexa Fluor® 488 conjugated Phalloidin, and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
687203 25 µg 76€
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687204 100 µg 236€
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Description

TP63 is a p53-related gene and shares similar domain organization with p53 and p73. Full length TP63 contains a N-terminal transactivation domain, a central DNA binding domain, and a C-terminal oligomerization domain responsible for the formation of a homotetramer. TP63 have several isoforms produced by alternative splicing. Most of the isoforms contain the DNA binding domain, but differ in their transactivation and oligomerization domains. The transactivation domain is present in the transcriptional active isoforms (TA-p63) and is absent in the truncated isoforms (ΔN -p63). TP63 is expressed in keratinocyte stem cells and is essential for the morphogenesis and development of ectodermal, orofacial, and limb tissues. TP63 deficient mice die soon after birth with skin and limb developmental defects.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human TP63 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 - 1:200 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml (1:250-1:1000 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Application Notes: This clone predominantly recognizes ΔN-p63, and weakly crosses react with TA-p63. Both  ΔN-p63 and TA-p63 have multiple isoforms, the largest isoforms are 66kD and 77kD respectively. This antibody recognizes more than one isoform of ΔN-p63.

RRID
AB_2721601 (BioLegend Cat. No. 687203)
AB_2721602 (BioLegend Cat. No. 687204)

Antigen Details

Structure
p53 family of transcription factors. 12 reported isoforms produced by alternative splicing. Predicted molecular weight ranged from 44.7kD to 76.8kD.
Distribution

Nuclear.

Function
TP63 acts as a transcriptional activator or repressor, is essential for limb formation, epidermal morphogenesis, and epithelial stem cell regeneration.
Interaction
Forms a homotetramer. Interacts with HIPK2, SSRP1, WWP1, PDS5A, and NOC2L.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Katoh I, et al. 2016. Cell Cycle 15:699.
2. Candi E, et al. 2014. Hum. Mutat. 35:702.
3. Chari NS, et al. 2013. Cell Death Differ. 20:1080.
4. Vasmatzis G, et al. 2012. Blood 120:2280.
5. Scott DW, et al. 2012. Blood 119:4949.
6. Miki D, et al. 2010. Nat. Genet. 42:893.

Gene ID
8626 View all products for this Gene ID
UniProt
View information about TP63 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/15/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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