GMP APC anti-human CD45RO Antibody, CD45RO, UCHL1

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Clone: UCHL1 (See other available formats)
Workshop: IV N31
Other Names: CD45RO
Isotype: Mouse IgG2a, κ
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Product Citations: publications

UCHL1_GMP_APC_CD45RO_Antibody_022322 — Typical results from human peripheral blood lymphocytes stained either with UCHL1 APC used at 5 μL/test (filled histogram) or with an isotype control (open histogram).

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260052

100 tests

281€

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Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details

Technical Data Sheet (pdf)

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

IL-2 dependent T cell line, CA1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

100 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁵ and formalin-fixed paraffin-embedded tissue sections⁴, Western blotting², and immunoprecipitation³.

Application References

Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
Yamada M, et al. 1996. Stroke 27:1155. (IHC)
Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

RRID

AB_2910361 (BioLegend Cat. No. 260052)

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure

Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)

Distribution

Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes

Function

Enhances TCR and BCR signaling

Ligand/Receptor

CD22

Cell Type

B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

CD Molecules

Antigen References

Thomas M. 1989. Annu Rev Immunol. 7:339-69.
Trowbridge I, et al. 1994. Annu Rev Immunol. 12:85-116.

Gene ID

5788 View all products for this Gene ID

UniProt

View information about CD45RO on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD45RO Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD45RO	UCHL1	FC
FITC anti-human CD45RO	UCHL1	FC
PE anti-human CD45RO	UCHL1	FC
PE/Cyanine5 anti-human CD45RO	UCHL1	FC
Purified anti-human CD45RO	UCHL1	FC,WB,IP,IHC-P
Alexa Fluor® 488 anti-human CD45RO	UCHL1	FC,IHC-P
Pacific Blue™ anti-human CD45RO	UCHL1	FC
Alexa Fluor® 700 anti-human CD45RO	UCHL1	FC
Biotin anti-human CD45RO	UCHL1	FC
Brilliant Violet 421™ anti-human CD45RO	UCHL1	FC,IHC-P
PerCP/Cyanine5.5 anti-human CD45RO	UCHL1	FC
Brilliant Violet 570™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 605™ anti-human CD45RO	UCHL1	FC
APC/Cyanine7 anti-human CD45RO	UCHL1	FC
PE/Cyanine7 anti-human CD45RO	UCHL1	FC
Brilliant Violet 650™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 711™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 785™ anti-human CD45RO	UCHL1	FC
Alexa Fluor® 594 anti-human CD45RO	UCHL1	IHC-P
Purified anti-human CD45RO (Maxpar® Ready)	UCHL1	FC,CyTOF®
Brilliant Violet 510™ anti-human CD45RO	UCHL1	FC
PE/Dazzle™ 594 anti-human CD45RO	UCHL1	FC
APC/Fire™ 750 anti-human CD45RO	UCHL1	FC
PerCP anti-human CD45RO	UCHL1	FC
APC anti-human CD45RO	UCHL1	FC
TotalSeq™-A0087 anti-human CD45RO	UCHL1	PG
TotalSeq™-B0087 anti-human CD45RO	UCHL1	PG
TotalSeq™-C0087 anti-human CD45RO	UCHL1	PG
Brilliant Violet 750™ anti-human CD45RO	UCHL1	FC
PE/Fire™ 640 anti-human CD45RO	UCHL1	FC
TotalSeq™-D0087 anti-human CD45RO	UCHL1	PG
Spark Violet™ 423 anti-human CD45RO	UCHL1	FC
GMP APC anti-human CD45RO	UCHL1	FC
PE anti-human CD45RO	UCHL1	FC
GMP PE anti-human CD45RO	UCHL1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/16/2022

For research use only. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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