- W19063E (See other available formats)
- Regulatory Status
- Other Names
- Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
- Rat IgG1, κ
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TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
AB_2922596 (BioLegend Cat. No. 376207)
AB_2922596 (BioLegend Cat. No. 376208)
- TNF superfamily; dimer/trimer; 17 kD (Mammalian)
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
- Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
- Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
- TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
- Cell Type
- B cells, Granulocytes, Lymphocytes, Macrophages, Monocytes, NK cells, NKT cells, T cells, Th1, Th17
- Biology Area
- Adaptive Immunity, Cell Death, Immunology, Innate Immunity
- Molecular Family
- Antigen References
- Volova L.T, et al. 2020. Biomolecules. 10:1563.
- Szondy Z, et al. 2017. Pharmacological Research. 115:124-132.
- Gene ID
- 7124 View all products for this Gene ID
- View information about TNF-alpha on UniProt.org
Related Pages & Pathways
Other FormatsView All TNF-α Reagents Request Custom Conjugation
|PE anti-human TNF-α||W19063E||ICFC|
|APC anti-human TNF-α||W19063E||ICFC|
|FITC anti-human TNF-α||W19063E||ICFC|
|PE/Cyanine7 anti-human TNF-α||W19063E||ICFC|
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