Direct-Blot™ HRP anti-RNA Polymerase II RPB3 Antibody

Pricing & Availability
Clone
1Y26 (See other available formats)
Regulatory Status
RUO
Other Names
RNA polymerase II sub-unit 3, RPB3, POLR2C
Isotype
Mouse IgG1
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Product Citations
publications
1Y26_DBHRP_RNAPolymeraseIIRPB3_Antibody_031519_updated.png
Total cell lysates (15 µg total protein) from HeLa and S. cerevisiae (yeast) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:1000 dilution of Direct-Blot™ anti-RPB3 Antibody, clone 1Y29, overnight at 4°C. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • 1Y26_DBHRP_RNAPolymeraseIIRPB3_Antibody_031519_updated.png
    Total cell lysates (15 µg total protein) from HeLa and S. cerevisiae (yeast) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:1000 dilution of Direct-Blot™ anti-RPB3 Antibody, clone 1Y29, overnight at 4°C. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
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665005 100 µL 232.00€
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Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The RNA polymermase II subunit RPB3 is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft and is known to be involved in transcription termination.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Yeast (Saccharomyces cerevisiae)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This clone is yeast RPB3-specific and should not be used to detect mammalian RPB3.

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation.

Application References
  1. Svetlov V, et al. 1998. J. Biol. Chem. 273:10827.
RRID
AB_2566702 (BioLegend Cat. No. 665005)

Antigen Details

Structure
RPB3 is a 45 kD subunit of the yeast RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
854791 View all products for this Gene ID
UniProt
View information about RNA Polymerase II RPB3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03/15/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-RNA Polymerase II RPB3

  • Direct-Blot™ HRP anti-RNA Polymerase II RPB3

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