Direct-Blot™ HRP anti-mCherry Antibody

Pricing & Availability
Clone
8C5.5 (See other available formats)
Regulatory Status
RUO
Other Names
DSRED
Isotype
Mouse IgG2a
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Product Citations
publications
85C.5_mCherry_WB_Image_010616
10 µg of total protein extract from 293E cells transfected with pcDNA3.1-mCherry was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-mCherry (clone 8C5.5). Proteins were visualized using chemiluminescence detection.
  • 85C.5_mCherry_WB_Image_010616
    10 µg of total protein extract from 293E cells transfected with pcDNA3.1-mCherry was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-mCherry (clone 8C5.5). Proteins were visualized using chemiluminescence detection.
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677703 100 µL 232.00€
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Description

mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins1,2. mCherry derives from proteins initially isolated from Cnidarians.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
mCherry.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2566703 (BioLegend Cat. No. 677703)

Antigen Details

Structure
236 amino acids with a predicted molecular weight of approximately 27 kD.
Function
Fluorescent protein.
Biology Area
Cell Biology
Antigen References

1. Shaner NC, et al. 2008. Nat. Methods 5:545.
2. Shaner NC, et al. 2005. Nat. Methods 2:905.

Gene ID
NA
UniProt
View information about mCherry on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 04/21/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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