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Direct-Blot™ HRP anti-Histone H3 Acetylated Lysine 9 (K9ac) Antibody

Pricing & Availability
Clone
2G1F9 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
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Product Citations
publications
2G1F9_DBHRP_K9ac_Antibody_1_060920
Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 5 mM sodium butyrate for 16 hours were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:2000 dilution of Direct-Blot™ HRP anti-Histone H3 K9ac antibody (clone 2G1F9) at room temperature for 2 hours. Proteins were visualized by chemiluminescence detection system. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • 2G1F9_DBHRP_K9ac_Antibody_1_060920
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 5 mM sodium butyrate for 16 hours were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:2000 dilution of Direct-Blot™ HRP anti-Histone H3 K9ac antibody (clone 2G1F9) at room temperature for 2 hours. Proteins were visualized by chemiluminescence detection system. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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698407 25 µL 100€
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698408 100 µL 272€
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability.
Description

Histone subunit H3, along with subunits 2A, 2B, and 4, make up the core subunits of the nucleosome octomer. An octomer contains two protomers of each subunit tightly wrapped around a ~147 bp segment of DNA. Histones have integral roles in chromatin integrity, genomic stability, and gene regulation. Post-translational modification of histones in response to certain stimuli results in alterations of nucleosomal positioning relative to DNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Histone H3 K9ac peptide
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:1000 - 1:2000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2861072 (BioLegend Cat. No. 698407)
AB_2861073 (BioLegend Cat. No. 698408)

Antigen Details

Structure
Histone H3 is a 134 aa protein with a predicted molecular weight of 15.4 kD. The subunit is comprised of a histone fold, an αN-helix main globular domain and a large N-terminal tail harboring residues subject to multiple types of post-translational.
Distribution

Nucleus

Function
Histone acetylation is generally associated with activation of gene expression, nucleosomal redistribution, and histone deposition. One consequence of this modification is the alteration of chromatin structure to facilitate recruitment of transcription factors. Additionally, acetyl-lysine modification is also used as binding site for proteins containing bromodomains, resulting in their recruitment to the modified histones.
Interaction
Histones 2A, 2B, 4, HDAC
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Zhang, et al. 2017. Nat. Commun. 8: 14799.
2. Kanda M, et al. 2016. Proc. Natl. Acad. Sci. USA 113: E3394.
3. Bellet MM, et al. 2013. Proc. Natl. Acad. Sci. USA 9: 3333.
4. Li B, et al. 2016. Sci. Rep. 6: 26542.
5. Li D, et al. 2014, Mol. Cancer. 13:26.

Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/09/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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