Direct-Blot™ HRP anti-GAPDH Antibody

Pricing & Availability
Clone
W17079A (See other available formats)
Other Names
glyceraldehyde-3-phosphate dehydrogenase
Isotype
Rat IgG2a, κ
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Product Citations
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W17079A_DB-HRP_GAPDH_Antibody_1_WB_062218
Whole cell lysates (15 µg protein) from HeLa and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:4000 Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Proteins were visualized using chemiluminescence detection. Lane M: MW ladder.
  • W17079A_DB-HRP_GAPDH_Antibody_1_WB_062218
    Whole cell lysates (15 µg protein) from HeLa and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:4000 Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Proteins were visualized using chemiluminescence detection. Lane M: MW ladder.
Cat # Size Price Quantity Avail. Save
607903 25 µl 68€
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607904 100 µl 232€
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Description

GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein (161-335 a.a.)
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 µg per ml (1:4000 - 1:16000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone weakly reacts with rat GAPDH.

RRID
AB_2734504 (BioLegend Cat. No. 607903)
AB_2734505 (BioLegend Cat. No. 607904)

Antigen Details

Structure
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution

Ubiquitously expressed, locate in cytoplasm and perinuclear region.

Function
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Interaction
Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
Biology Area
Cell Biology, Western Blot Controls
Antigen References
  1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
  2. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460.
  3. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527.
  4. Zheng L, et al. 2003. Cell. 114:255.
  5. Wang Q, et al. 2005. FASEB J. 19:869.
  6. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
Gene ID
5590 View all products for this Gene ID
UniProt
View information about GAPDH on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/22/2018

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