Direct-Blot™ HRP anti-6-His Epitope Tag Antibody

Pricing & Availability
Clone
6-His (See other available formats)
Regulatory Status
RUO
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
6-His_DB-HRP_6-His_Epitope_Tag_Antibody_051118
Western blot analysis of 15 µg cell lysates from HeLa cells transfected with (+) or without (−) His-tagged plasmid. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.05, 0.1 and 0.5 µ/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-His-HRP antibody, clone 6-His. Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
  • 6-His_DB-HRP_6-His_Epitope_Tag_Antibody_051118
    Western blot analysis of 15 µg cell lysates from HeLa cells transfected with (+) or without (−) His-tagged plasmid. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.05, 0.1 and 0.5 µ/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-His-HRP antibody, clone 6-His. Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
Cat # Size Price Quantity Check Availability Save
906109 25 µL 76.00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
906110 100 µL 232.00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
6xHis Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Purity
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 5.0 µg per ml (1:1000-1:10000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References
  1. Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed
  3. Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (ICC)
  5. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
  6. Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed
  7. Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
RRID
AB_2734681 (BioLegend Cat. No. 906109)
AB_2734682 (BioLegend Cat. No. 906110)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/12/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account