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Brilliant Violet 711™ anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
OKT4_BV711_061812
Human peripheral blood lymphocytes were stained with CD4 (clone OKT4) Brilliant Violet 711™.
  • OKT4_BV711_061812
    Human peripheral blood lymphocytes were stained with CD4 (clone OKT4) Brilliant Violet 711™.
Compare all formats See Brilliant Violet 711™ spectral data
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317439 25 tests 159€
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317440 100 tests 344€
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  2. Wang X, et al. 2022. Nat Commun. 13:4823. PubMed
  3. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
  4. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  5. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  6. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  7. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  8. Bending D, et al. 2014. J Immunol. 193:2699. PubMed
  9. Dart SJ, et al. 2021. Sci Rep. 11:15312. PubMed
  10. Haruna M, et al. 2020. Biol Pharm Bull. 43:399. PubMed
  11. Alter G, et al. 2020. Cell. 183(1):185-196.e14. PubMed
  12. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  13. Sahillioglu AC, et al. 2021. Cancer Immunol Res. 9:999. PubMed
  14. Weaver JD, et al. 2022. Oncoimmunology. 11:2141007. PubMed
  15. Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed
  16. Mengrelis K, et al. 2021. Pediatr Transplant. 25:e13930. PubMed
  17. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  18. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  19. Webb K et al. 2018. Frontiers in immunology. 2.574305556 . PubMed
  20. Bradley T, et al. 2020. Nat Commun. 11:948. PubMed
  21. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  22. Han Q, et al. 2020. Cell Rep. 30:1553. PubMed
  23. Antonarakis ES, et al. 2018. Clin Cancer Res. 24:4662. PubMed
  24. Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed
  25. Bending D, et al. 2015. J Immunol. 195: 5616 - 5624. PubMed
  26. Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed
  27. Aid M et al. 2017. Cell. 169(4):610-620 . PubMed
  28. Tong L, et al. 2021. Front Microbiol. 12:704449. PubMed
  29. van Stigt Thans T, et al. 2020. Journal of Virology. 93(20):e00719-19.. PubMed
  30. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  31. Borducchi E, et al. 2016. Nature. 540:284-287. PubMed
  32. Paquin-Proulx D, et al. 2016. J Immunol. 197: 1843 - 1851. PubMed
RRID
AB_11219404 (BioLegend Cat. No. 317439)
AB_2562912 (BioLegend Cat. No. 317440)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 4    Revision Date: 07/13/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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