Brilliant Violet 605™ anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_BV605_CD45_Antibody_FC_080113.jpg
C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 605™ (filled histogram) or rat IgG2b, κ Brilliant Violet 605™ isotype control (open histogram).
  • 30-F11_BV605_CD45_Antibody_FC_080113.jpg
    C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 605™ (filled histogram) or rat IgG2b, κ Brilliant Violet 605™ isotype control (open histogram).
See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Check Availability Save
103139 125 µL 156.00€
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103155 50 µg 204.00€
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103140 500 µL 316.00€
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining with µg, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For flow cytometric staining with µl, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Kakizaki M, Watanabe R 2017. Neuropathology. 10.1111/neup.12386. PubMed
  2. Pavelko K, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01532. PubMed
  3. García Nores GD, et al. 2018. Nat Commun. 9:1970. PubMed
  4. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  5. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  6. Chen JC et al. 2018. Cell systems. 7(1):92-103 . PubMed
  7. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  8. Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed
  9. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  10. Freemerman AJ, et al. 2019. J Immunol. 202:1265. PubMed
  11. Gkountela S et al. 2019. Cell. 176(1-2):98-112 . PubMed
  12. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  13. Bennion BG, et al. 2019. J Virol. 93. PubMed
  14. Zhang Y, et al. 2019. Nat Commun. 10:3667. PubMed
  15. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  16. Dalmas E et al. 2017. Immunity. 47(5):928-942 . PubMed
  17. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  18. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  19. Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed
  20. McDonald LT, et al. 2017. PLoS One. 12:e0180724. PubMed
  21. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  22. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  23. Lloyd AF, et al. 2019. Nat Neurosci. 1.643055556. PubMed
  24. Shao Y, et al. 2019. Stem Cells. 37:1331. PubMed
  25. Gyoneva S, et al. 2019. Life Sci Alliance. 2:e201900453. PubMed
  26. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  27. Wagner JA, et al. 2020. Cell Rep. 31:107720. PubMed
  28. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  29. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  30. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  31. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  32. Berrien–Elliott MM, et al. 2020. Immunity. 51(3):479-490. PubMed
  33. Chen YG, et al. 2020. Molecular Cell. 76(1):96-109. PubMed
  34. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  35. Dulken BW, et al. 2019. Nature. 571:205. PubMed
  36. Shao Y, et al. 2019. Diabetes. 68:2131. PubMed
  37. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  38. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  39. Payne K, et al. 2016. J Leukoc Biol. 100: 625 - 635. PubMed
  40. Cortez-Toledo O, et al. 2017. PLoS One. 12(2):e0171268. PubMed
  41. Kuhn NF, et al. 2020. Nat Commun. 4.74375. PubMed
  42. Zou C, et al. 2020. Cell Rep. 33:108447. PubMed
  43. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  44. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  45. Myers DR, et al. 2020. Front Immunol. 11:576310. PubMed
  46. Wong P, et al. 2021. STAR Protoc. 2:100262. PubMed
  47. Marinaccio C, et al. 2021. Cancer Discovery. 11(6):1398-1410. PubMed
  48. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
  49. Becattini S, et al. 2021. Cell Host Microbe. 29(3):378-393.e5. PubMed
  50. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  51. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  52. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  53. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  54. Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed
  55. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  56. Lu SX, et al. 2021. Cell. . PubMed
  57. Israelow B, et al. 2020. bioRxiv. . PubMed
  58. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  59. Gao Y, et al. 2020. Nat Cell Biol. 1064:22. PubMed
  60. Lee J, et al. 2020. Gut Microbes. 1:. PubMed
  61. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  62. Perez-Cruz M, et al. 2021. PLoS One. e0236216:16. PubMed
  63. Molgora M, et al. 2020. Cell. 182:886. PubMed
  64. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  65. Nording H, et al. 2021. Nat Commun. 12:3352. PubMed
  66. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
RRID
AB_2562341 (BioLegend Cat. No. 103139)
AB_2650656 (BioLegend Cat. No. 103155)
AB_2562342 (BioLegend Cat. No. 103140)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/11/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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