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Brilliant Violet 510™ anti-human CD154 Antibody

Pricing & Availability
Clone
24-31 (See other available formats)
Regulatory Status
RUO
Other Names
CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
24-31_BV510_CD154_Antibody_FC_111113
PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were stained with CD154 (clone 24-31) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).
  • 24-31_BV510_CD154_Antibody_FC_111113
    PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were stained with CD154 (clone 24-31) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).
Compare all formats See Brilliant Violet 510™ spectral data
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310829 25 tests 172€
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310830 100 tests 348€
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Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3 and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF)
  2. Rushworth SA, et al. 2002. Transplantation 73:635. (Block)
  3. Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF)
  4. Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block)
  5. Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed
  6. Kuchen S, et al. 2007. J. Immunol. 179:5886.
  7. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
RRID
AB_2562715 (BioLegend Cat. No. 310829)
AB_2563833 (BioLegend Cat. No. 310830)

Antigen Details

Structure
TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution

Activated T cells

Function
B cell costimulation
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.

Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/15/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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